目的 觀察坤泰膠囊對(duì)卵巢早衰模型大鼠的治療作用，并初步探討其作用機(jī)制。方法 將40只SD雌性大鼠隨機(jī)分為4組：對(duì)照組、模型組、坤泰膠囊組、結(jié)合雌激素片組，除對(duì)照組外，ig 75 mg/kg雷公藤多苷片制備卵巢早衰模型。造模結(jié)束后，坤泰膠囊和結(jié)合雌激素片組分別以0.6、0.625 g/kg劑量ig給藥，連續(xù)給藥36 d。HE染色觀察卵巢形態(tài)并進(jìn)行卵泡計(jì)數(shù)；采用酶聯(lián)免疫吸附（Elisa）法檢測(cè)卵泡刺激素（FSH）、黃體生成素（LH）、血清雌二醇（E₂）、及抗繆勒管激素（AMH）水平；實(shí)時(shí)熒光定量PCR（qRT-PCR）法檢測(cè)卵巢組織中血管內(nèi)皮生長(zhǎng)因子（VEGF）、堿性成纖維細(xì)胞生長(zhǎng)因子（bFGF）mRNA的表達(dá)變化。結(jié)果 與對(duì)照組比較，模型組卵巢內(nèi)的始基卵泡、竇前卵泡、竇狀卵泡數(shù)目明顯減少，閉鎖卵泡數(shù)目明顯增多（P<0.05）；E₂、AMH水平明顯降低（P<0.05），F(xiàn)SH、LH水平明顯升高（P<0.05）；VEGF、bFGF mRNA水平均明顯降低（P<0.05）。與模型組比較，坤泰膠囊組卵巢內(nèi)的始基卵泡、竇狀卵泡、竇前卵泡數(shù)目明顯增多，閉鎖卵泡數(shù)目明顯減少（P<0.05）；E₂、AMH水平明顯升高（P<0.05），F(xiàn)SH、LH水平明顯降低（P<0.05）；VEGF、bFGF mRNA水平均明顯升高（P<0.05）。結(jié)論 坤泰膠囊通過調(diào)節(jié)激素水平及上調(diào)VEGF、bFGF mRNA表達(dá)，修復(fù)受損的卵巢組織，促進(jìn)卵泡的生長(zhǎng)發(fā)育，從而抑制卵泡過早耗竭。

Objective To explore the effect and mechanism of Kuntai Capsule (KC) on premature ovarian failure in rats. Methods Totally 40 SD female rats were randomly divided into four groups:control group, model group, Kuntai Capsule (KC) group and conjugated estrogens tablets (CET) group. The premature ovarian failure model in rats was made by ig administration of 75 mg/kg tripterygium. After the model was established, rats were continually treated with 0.6 and 0.625 g/kg of KC and CET respectively by ig administration for 36 d. HE staining was used to observe the morphology of ovary and count the number of follicle. The enzyme-linked immunosorbent assay (Elisa) method was used for the detection of follicle stimulating hormone (FSH), luteinizing hormone (LH), serum estradiol (E₂), and anti-Mueller tube hormone (AMH) level. The real-time fluorescence quantitative PCR (qRT-PCR) method was used to detect the mRNA expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF). Results Compared with control group, the number of primordial follicles, preantral follicles and antral follicles were significantly reduced, the number of atretic follicles was increased (P<0.05), E₂ and AMH significantly decreased (P<0.05), FSH and LH levels increased significantly (P<0.05), and the levels of VEGF and bFGF mRNA in model group were significantly decreased (P<0.05). Compared with model group, the number of primordial follicles, preantral follicles, and antral follicles were significantly increased, the number of atretic follicles was significantly reduced (P<0.05), E₂ and AMH significantly increased (P<0.05), FSH and LH levels significantly decreased (P<0.05), and the levels of VEGF and bFGF mRNA in KC group were significantly increased (P<0.05). Conclusion By regulating the level of hormone and up-regulating the expression of VEGF and bFGF, KC can repair the damaged ovarian tissue and promote the growth and development of the follicle, so as to inhibit the premature exhaustion of mRNA.

國(guó)家中醫(yī)藥管理局高慧全國(guó)名老中醫(yī)藥專家傳承工作室建設(shè)項(xiàng)目（2015048157）；河北省中醫(yī)藥管理局中醫(yī)藥類科研計(jì)劃課題（2016068）；河北省省級(jí)重大醫(yī)學(xué)科研項(xiàng)目（zyzd2013018）；河北省政府資助臨床醫(yī)學(xué)優(yōu)秀人才培養(yǎng)項(xiàng)目（2014571024）