目的采用幼齡大鼠慢性腹瀉模型,評價小兒止瀉膏的止瀉作用,并探討其作用機制。方法采用ig番瀉葉制備幼齡大鼠慢性腹瀉模型,給予蒙脫石散1.62 g/kg和小兒止瀉膏低、中、高劑量（2.03、4.05、8.10 g/kg）進(jìn)行治療,給藥1、3 d,分別測定稀便率、稀便級、腹瀉指數(shù)；給藥第4天,測定小腸含水量并取血,比色法測定血清中琥珀酸脫氫酶（SDH）、淀粉酶活力、D-木糖,酶聯(lián)免疫法測定血清D-乳酸、白介素-1β（IL-1β）及腫瘤壞死因子-α（TNF-α）水平。結(jié)果給藥1次,小兒止瀉膏4.05、8.10 g/kg劑量組均可明顯降低稀便率,8.10 g/kg劑量組明顯降低腹瀉指數(shù)；給藥3次,小兒止瀉膏2.03、4.05、8.10 g/kg劑量組均能明顯降低稀便率,4.05、8.10 g/kg劑量組明顯降低腹瀉指數(shù),降低血清D-乳酸水平,8.10 g/kg劑量組能明顯升高血清D-木糖水平。小兒止瀉膏對SDH、淀粉酶活力及IL-1β、TNF-α水平均無明顯影響。結(jié)論小兒止瀉膏對幼齡大鼠慢性腹瀉有明顯的治療作用,主要作用機制是改善腸道的吸收功能及通透性。

Objective To evaluate the therapeutic effect of Xiao'er Zhixie Paste (XZP) by using the young rat model of chronic diarrhea, and to explore its mechanism. Methods Chronic diarrhea model in young rats was induced by ig senna. Rats were ig with Montmorillonite powder of 1.62 g/kg, XZP of low, medium, and high dose (2.03, 4.05, and 8.10 g/kg) for treatment. Loose stools rate, loose stool grade and diarrhea index were determined 1 and 3 d after treatment respectively. The water content of small intestine was measured and blood was collected for testing serum succinate dehydrogenase (SDH), amylase, D-xylose by colorimetric determination, testing serum D-lactic acid, IL-1β, and TNF-α by Elisa after administration. Results The rate of loose stools in XZP 4.05 and 8.10 g/kg dose group, and diarrhea index in 8.10 g/kg dose group significantly reduced after the first treatment. The loose stools rate of XZP 2.03, 4.05, and 8.10 g/kg dose group, diarrhea index, serum D-lactic acid level in 4.05, 8.10 g/kg group significantly reduced, and serum D-xylose level in 8.10 g/kg dose group significantly increased 3 d after treatment. However, XZP had no significant effect on SDH, amylase activity and IL-1β, TNF-α levels. Conclusion XZP has obvious therapeutic effect on chronic diarrhea in young rats, the mechanism is to increase improve the absorptive function and permeability of intestinal tract.