口服藥物腸道吸收的研究方法主要包括體外法、體內(nèi)法和在體法，對(duì)這3種研究方法進(jìn)行簡(jiǎn)要介紹。鑒于在體腸灌流法操作簡(jiǎn)便、技術(shù)成熟、可控性強(qiáng)，同時(shí)又保證了神經(jīng)內(nèi)分泌調(diào)節(jié)與淋巴液血液供應(yīng)的完整性等特點(diǎn)，更能反映藥物吸收的真實(shí)情況，因此重點(diǎn)介紹了在體腸灌流法，并對(duì)在體腸灌流法中使用的循環(huán)灌流法和單向灌流法常用的幾種灌流液體積校正方法等進(jìn)行綜述，為研究口服藥物腸道吸收試驗(yàn)設(shè)計(jì)提供借鑒和參考。為保證試驗(yàn)的可操作性和實(shí)驗(yàn)結(jié)果的準(zhǔn)確性，認(rèn)為應(yīng)根據(jù)所研究藥品的性質(zhì)、實(shí)驗(yàn)要求、試驗(yàn)條件等多方面因素綜合考慮，選擇適宜的試驗(yàn)方法，進(jìn)而為藥物劑型的開發(fā)和臨床合理用藥提供依據(jù)。

At present, the study of intestinal absorption of oral drugs mainly includes in vitro, in vivo and in situ methods. In view of the advantages of in situ intestinal perfusion such as simple operation, mature technology, controllable, ensure the neuroendocrine regulation and blood supply, and so on, which could better reflect the true situation of drug absorption. In this study, the research methods and characteristics of intestinal absorption of oral drugs were systematically introduced. The recirculating perfusion method and single-pass perfusion method were compared, and several volume correction methods were also introduced. In order to ensure the operability and accuracy of experimental results, proper experiment method of intestinal absorption will be adopt according to the factors such as drug characters, experiment requirements, experimental conditions, and so on. The article provides a scientific basis for the development of pharmaceutical dosage and clinical rational drug use.

遼寧省科學(xué)技術(shù)計(jì)劃項(xiàng)目（2014226033）