目的 研究不同產(chǎn)地枇杷葉醇提物對抗炎靶標磷酸二酯酶4（PDE4）的抑制作用。方法 采用體積分數(shù)95%乙醇回流提取法提取藥材，以標記液體閃爍計數(shù)法檢測提取物對PDE4的D2亞型靶標（PDE4D2）的抑制活性。結(jié)果 測試體系的藥物終質(zhì)量濃度為5 mg/L時，20個樣品中9個測試樣品的抑制率在80%以下；11個樣品的抑制率大于80%，其中8個樣品大于90%。結(jié)論 不同地枇杷葉醇提物抑制PDE4靶標的活性存在明顯差異。

Objective To investigate the inhibition of Eriobotryae Folium from twenty different districts towards phosphodiesterase 4(PDE4) in vitro. Methods The Eriobotryae Folium were extracted with 95% ethanol reflux and the inhibition rates against PDE4D2 were carried out by liquid scintillation counting method. Results All the samples exhibited inhibitory activities towards PDE4 at 5 mg/L. Among them, nine samples were of the inhibition rate less than 80%, eleven samples were of more than 80% inhibition and eight samples were of more than 90% inhibition. Conclusion The Eriobotryae Folium shows significantly different inhibitory activities towards PED4.

國家自然科學(xué)基金項目（21103234，81373258）；廣東自然科學(xué)基金團隊項目（S2011030003190）；廣州市科技計劃項目（201607010293，2014J4100165）；國家公益性行業(yè)專項（201407002）