目的 建立酸棗葉藥材的HPLC指紋圖譜，為其質(zhì)量標(biāo)準(zhǔn)的研究提供依據(jù)。方法 采用Kinetex C₁₈色譜柱（100 mm×2.1 mm，2.6 μm）；流動(dòng)相為乙腈-0.1%磷酸酸水溶液，梯度洗脫；體積流量為300 μL/min；柱溫30℃，檢測波長225 nm，進(jìn)樣量10 μL，對(duì)12批酸棗葉藥材進(jìn)行了指紋圖譜研究，采用中藥色譜指紋圖譜相似度評(píng)價(jià)系統(tǒng)（2012版）軟件進(jìn)行分析。結(jié)果 12批酸棗葉的HPLC指紋圖譜有13個(gè)共有峰，其中1個(gè)共有峰得到確認(rèn)，相似度均＞0.85。結(jié)論 該方法準(zhǔn)確可靠，重復(fù)性好，為更好地控制酸棗葉內(nèi)在質(zhì)量提供科學(xué)依據(jù)。

Objective To establish a specific HPLC method and a standard fingerprint for quality control of Ziziphi Spinosae Folium. Methods The samples were separated on a Kinetex C₁₈ column (100 mm ×2.1mm, 2.6 μm) by gradient elution at the flow rate of 300 μL/min using acetonitrile and 0.1% aqueous formic acid as the mobile phase. The column temperature was 30℃. The detection wavelength was 225 nm and the sample size was 10 μL. The fingerprint evaluation software (2012 edition) for Chinese materia medica (CMM) was used to evaluate the similarity of the 12 batches of samples. Results There were 13 characteristic peaks identified in the characteristic spectra of Ziziphi Spinosae Folium samples.Peak 6 was Rutin.The similarities of 13 batches of Ziziphi Spinosae Folium samples were proved to be higher than 0.850. Conclusion The method is available with a good reproducibility and accuraty which can control the quality standards effectively.

河北省自然基金研究項(xiàng)目（H2015423026）；河北省高等學(xué)?？茖W(xué)技術(shù)研究項(xiàng)目（ZD2015001）；河北省中醫(yī)藥管理局科研計(jì)劃項(xiàng)目（2017013）