目的 建立降植烷誘導(dǎo)的系統(tǒng)性紅斑狼瘡（SLE）小鼠模型，并對模型進(jìn)行全面驗證。方法 6~8周齡雌性BALB/c小鼠隨機(jī)分為兩組，模型組單次ip降植烷0.5 mL，對照組單次ip生理鹽水0.5 mL。注射前及注射后2、3、4、5、6個月ELISA法檢測血清中抗SLE抗體（anti-SLE）、抗雙鏈DNA抗體（anti-dsDNA）；注射前及注射后每月1次采用目測尿蛋白試紙測定小鼠尿蛋白；6個月處死動物，觀察腎臟HE染色及直接免疫熒光染色后鏡下變化。結(jié)果 小鼠ip降植烷2個月后，其自身抗體（anti-dsDNA、anti-SLE）濃度顯著高于對照組（P<0.05、0.01），且6個月內(nèi)抗體濃度逐漸升高；6個月時，73%模型組小鼠出現(xiàn)++++尿蛋白；腎臟HE切片顯示模型組小鼠腎臟出現(xiàn)腎小球腫脹、炎癥細(xì)胞浸潤等典型的腎病理改變，直接免疫熒光染色見模型組小鼠腎小球毛細(xì)血管存在免疫復(fù)合物沉積，對照組小鼠腎臟組織未見改變。結(jié)論 降植烷成功誘導(dǎo)SLE動物模型。

Objective To establish the systemic lupus erythematosus (SLE) mouse model through pristaneip injection and validate the model comprehensively. Methods Female BALB/c mice of 6-8 weeks were randomly divided into two groups. Animals in model group were injected with 0.5 mL pristane by ip injection while in control group with 0.5 mL normal saline. Anti-systemic lupus erythematosus antibodies (anti-SLE) and anti-double strand DNA antibodies (anti-dsDNA) were checked before injection and monthly thereafter. Proteinuria was detected before injection and every month thereafter. All mice were bled to death 6 months after injection. Kidneys were excised to observe the histopathologic evidence of glomerulonephritis. Results The concentration of anti-dsDNA and anti-SLE antibody in sera was higher of model group than that of control group two months after pristane injection, and the concentration of antibody gradually increased within 6 months. At the sixth months, the protein concentration of urine in most model group mice was ++++. The histopathology and imunoflorescence of kidney sections indicated typical evidence of glomerulonephritis in model group. Conclusion The murine lupus model can be successfully established in female BALB/c mouse with a single ip injection of 0.5mL of pristane.

黑龍江省教育廳科學(xué)技術(shù)研究項目（12541219）