目的 研究氧化苦參堿在體外對(duì)人肺癌細(xì)胞（A549）、人肝癌細(xì)胞（HepG-2）增殖抑制與促凋亡活性，并進(jìn)行比較。方法 通過MTT比色法和生長(zhǎng)曲線法比較氧化苦參堿對(duì)兩種腫瘤細(xì)胞增殖活性的抑制作用，采用HE染色、Hoechst 33258熒光染色和透射電鏡3種方法比較氧化苦參堿對(duì)兩種腫瘤細(xì)胞形態(tài)的影響及促凋亡作用。結(jié)果 MTT結(jié)果顯示，與對(duì)照組比較，氧化苦參堿200、400、800 μg/mL濃度組A549、HepG-2細(xì)胞存活率均顯著降低（P<0.05、0.01），半數(shù)抑制濃度（IC₅₀）分別為1 055.45、774.11 μg/mL；生長(zhǎng)曲線結(jié)果顯示，與對(duì)照組比較，氧化苦參堿組HepG-2細(xì)胞培養(yǎng)第4、5天及A549細(xì)胞第5天細(xì)胞數(shù)顯著下降（P<0.05、0.01）；在HE染色、Hoechst 33258熒光染色和透射電鏡形態(tài)學(xué)觀察實(shí)驗(yàn)中，氧化苦參堿對(duì)HepG-2細(xì)胞作用較明顯，細(xì)胞數(shù)量明顯減少，同時(shí)出現(xiàn)細(xì)胞絨毛減少、細(xì)胞核變小變圓、固縮等明顯的細(xì)胞凋亡形態(tài)學(xué)特征，對(duì)A549細(xì)胞形態(tài)的影響較弱。結(jié)論 氧化苦參堿具有抑制腫瘤細(xì)胞增殖和促進(jìn)凋亡的作用，對(duì)HepG-2作用較強(qiáng)，對(duì)A549作用較弱，具有一定的特異性。

Objective To study the proliferative inhibition and apoptosis promoting activity of oxymatrine on human lung cancer cells (A549) and human hepatocellular carcinoma cells (HepG-2) in vitro, and make a comparison. Methods Effect of oxymatrine on the proliferation activity of two kinds of tumor cells were compared by MTT method and growth curve method. HE staining, Hoechst 33258 fluorescent staining and transmission electron microscopy were used to compare morphological changes. Results MTT results showed that, compared with control group, the survival rate of oxymatrine 200, 400, and 800 μg/mL concentration group in A549 and HepG-2 cells decreased significantly (P < 0.05, 0.01), half inhibitory concentration (IC₅₀) were 1055.45 and 774.11 g/mL respectively; The growth curve showed that, compared with control group, the number of cells in oxymatrine group of HepG-2 cells cultured for fourth and fifth day and A549 cells cultured for fifth day decreased significantly (P < 0.05, 0.01). Oxymatrine affected morphological changes of HepG-2 cells more significantly than A549 cells. The number of cells in the oxymatrine group was significantly reduced; And the cell villi was decreased, the nucleus was small and round, and the cell apoptosis morphological characteristics were obvious. Conclusion Oxymatrine can inhibit the proliferation cells and promote the apoptosis of tumor cells. It affected HepG-2 cells more obviously, which suggests that it may have different sensitivity to different tumor cells.

哈爾濱市科技創(chuàng)新人才研究專項(xiàng)基金資助（2016RQQXJ095）；哈爾濱商業(yè)大學(xué)大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃國(guó)家級(jí)項(xiàng)目（201710240001）