目的 利用正交試驗(yàn)結(jié)合體外模型測(cè)定固公果根總?cè)疲═TROG）提取物抑制大鼠離體腸平滑肌收縮活性，優(yōu)化其提取工藝。方法 以熊果酸為對(duì)照品，采用香草醛-冰醋酸顯色，紫外分光光度法測(cè)定TTROG提取物中三萜含量，單因素考察提取溶劑、料液比、提取溫度、提取時(shí)間、提取次數(shù)對(duì)TTROG提取率的影響；在單因素實(shí)驗(yàn)的基礎(chǔ)上采用正交試驗(yàn)法考察提取溶劑、料液比、提取時(shí)間對(duì)TTROG提取率的影響；通過(guò)張力傳感器與BL-420生物實(shí)驗(yàn)多通道生理信號(hào)采集處理系統(tǒng)記錄不同提取物對(duì)體外腸平滑肌收縮的抑制作用，利用加權(quán)方法使生物活性與提取率相結(jié)合評(píng)價(jià)TTROG的提取工藝。結(jié)果 TTROG的最佳提取工藝條件為：提取溶劑80%乙醇，料液比1：10，提取時(shí)間2 h，提取溫度80℃，提取次數(shù)3次；最優(yōu)條件下TTROG的提取率可達(dá)42.12 mg/g；TTROG提取物對(duì)十二指腸、空腸、回腸、結(jié)腸4個(gè)腸段都具有顯著的抑制離體腸平滑肌收縮的作用，呈劑量相關(guān)性，對(duì)空腸的作用最強(qiáng)，抑制率可達(dá)41.96%。結(jié)論 經(jīng)優(yōu)化后的TTROG提取工藝提取率高、穩(wěn)定，且獲取的TTROG提取物具有顯著的抑制大鼠離體腸平滑肌收縮功能。

Objective To optimize the extraction technology of total triterpenoid from root of Rose odorata var. gigantean (TTROG) by orthogonal test combined with the contraction effect of TTROG on the isolated intestinal smooth muscle of rats in vitro. Methods UV spectrophotometric method was used to determine the contents of total triterpenoids in the TTROG extractive at the wavelength of 550 nm by taking ursolic acid as standard substance, and vanillin acetic acid as chromogenic reagent. The extraction rate of total triterpenoids was used as index to evaluate the technology based on single factor test, in which three factors were considered as follows:the concentration of extraction solvent, ratio of material to liquid, extraction time, and their interaction on extraction were studied by orthogonal experimental design. The inhibition effect of different extracts obtained from the optimized extraction process on the contraction of intestinal smooth muscle were recorded by tension transducer to the BL-420 biological experimental multi-channel physiological signal acquisition and processing system. The extraction process of TTROG was evaluated by the combination of biological activity and extraction rate with weighting method. Results The optimal extraction conditions of TTROG were as follows:extraction solvent 80% ethanol, solid-liquid ratio 1:10, extraction time for 2 h, three times and extraction temperature of 80℃. The optimized extraction rate could reach 42.12 mg/g. TTROG obtained using the optimized method showed significantly contraction effect on rat intestinal smooth muscle with dose effect dependence, and the effect on jejunum was the strongest, and the inhibition rate was 41.96%. Conclusion The optimized extraction technology is stable and effective with high extraction rate. TTROG showed the significant inhibitory function on contraction of isolated rat intestinal smooth muscle.

國(guó)家自然科學(xué)基金項(xiàng)目（81673693）