目的 研究注射用益氣復(fù)脈（凍干，YQFM）對¹³⁷Cs輻照后小鼠免疫系統(tǒng)的影響。方法 按體質(zhì)量將昆明小鼠隨機(jī)分為5組：對照組、模型組和YQFM低、中、高劑量（354.55、709.09和1 418.18 mg/kg）組，每組10只。除對照組外，其余各組小鼠放在圓形有孔的照射盒中采用¹³⁷Cs全身1次性照射劑量為3 Gy的射線，制備免疫低下小鼠模型，于照射24 h后，尾iv給藥，每天1次，連續(xù)6 d，對照組和模型組尾iv同體積輔料溶液。檢測小鼠胸腺指數(shù)；ip 5%的雞紅細(xì)胞懸液檢測血清溶血素的含量；耳廓遲發(fā)型超敏反應(yīng)模型采用2，4-二硝基氯苯制備，以小鼠耳腫脹度為觀測指標(biāo)；采用動物血液分析儀測定小鼠骨髓有核細(xì)胞（BMNC）和白細(xì)胞（WBC）數(shù)；用全自動生化儀測定血清中尿素氮（BUN）和丙氨酸轉(zhuǎn)氨酶（ALT）的含量變化。結(jié)果 與模型組比較，YQFM各劑量組均可顯著增加免疫低下小鼠的胸腺指數(shù)、顯著增強(qiáng)遲發(fā)型超敏反應(yīng)和增加BMNC、WBC數(shù)（P<0.05、0.01）；低、高劑量組顯著增加血清溶血素水平（P<0.01）；低劑量組顯著減少血清中ALT含量（P<0.05）。結(jié)論 YQFM可顯著改善¹³⁷Cs所致的小鼠免疫低下，有可能成為一種新型的抗腫瘤輔助用藥。

Objective To study the effect of Yiqi Fumai Lyophilized Injection (YQFM) on immune system of mice after exposure to ¹³⁷Cs irradiation. Methods KM mice were divided into five groups:control group, model group, YQFM low, medium, high dose (354.55, 709.09 and 1 418.18 mg/kg) groups, with 10 mice in each group. Except for control group, mice in remaining groups were placed in a circular perforated irradiation box using ¹³⁷Cs systemic irradiation dose of 3 Gy radiation, preparing immune compromised mouse model. After 24 h exposure, drugs were administrated in tail vein continued for six days, once a day. Mice in control and model group were injected with the same volume of intravenous infusion solution. Thymus index of mice was detected. Detection of serum hemolysin level was taken by ip injection of 5% chicken erythrocyte suspension. Using 2, 4-dinitrochlorobenzene to establish the delayed type hypersensitivity model, and the changes of ear swelling degree were observed. Moreover, the number of bone marrow nucleated cells (BMNC) and leucocyte (WBC) in mice were determined by animal blood analyzer. Change of the level of serum Urea nitrogen (BUN) and alanine amino transferase (ALT) were measured by automatic biochemical analyzer. Results Compared with model group, low, medium and high doses of YQFM significantly increased the thymus index in immune compromised mice, and significantly enhanced delayed type hypersensitivity and increased the number of BMNC and WBC (P<0.05、0.01); YQFM at the low and high doses increased the production of hemolysin; Low dose of YQFM significantly decreased the level of serum ALT (P<0.05). Conclusion YQFM can markedly enhance the immune function in mice with immunodeficiency induced by ¹³⁷Cs, and is expected to become a novel antineoplastic assistant drug.

天津市中藥注射劑關(guān)鍵技術(shù)校企協(xié)同創(chuàng)新實(shí)驗(yàn)室建設(shè)基金（17PTSYJC00090）