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[摘要]
目的 研究注射用益氣復脈(凍干)的化學成分。方法 采用超高效液相色譜儀-質譜(UPLC-Q-TOF/MS)方法對注射用益氣復脈(凍干)成分進行分析,通過各色譜峰的質譜數(shù)據(jù)與Waters UNIFI數(shù)據(jù)庫匹配,并結合二級碎片的裂解規(guī)律和文獻報道進行解析。結果 從注射用益氣復脈(凍干)中共鑒定出145個化合物,其中有26個藥效成分為首次檢出,包括17個黃酮類化合物,4個麥冬皂苷類化合物以及5個木脂素類化合物。結論 UPLC-Q-TOF/MS法可以快速、有效的鑒別注射用益氣復脈(凍干)的相關成分,為中藥質量控制提供依據(jù)。
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[Abstract]
Objective To study the chemical constituents of Yiqi Fumai Lyophilized Injection. Methods The constituents of Yiqi Fumai Lyophilized Injection were analyzed by UPLC-Q-TOF/MS. The mass spectra of each peak were matched with the Waters UNIFI database, and the cracking rules of the second-level fragments and related literatures were used for identification. Result A total of 145 compounds were identified. Therein 26 active ingredients were first reported from Yiqi Fumai Lyophilized Injection, including 17 flavonoids, 4 ophiopogon japonicosides and 5 lignans. Conclusion UPLC-Q-TOF/MS can be used to identify the active ingredients of Yiqi Fumai Lyophilized Injection rapidly and efficiently, and the result can be provided a basis for TCM quality control.
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