目的 探討刺五加多糖（ASPs）對刀豆蛋白A（Con A）造成的免疫性肝損傷模型的保護(hù)作用。方法 將BALB/c小鼠隨機(jī)分成7組，對照組，模型組，ASPs低、中、高劑量（36.25、72.50、145.00 mg/kg）組，聯(lián)苯雙酯滴丸（陽性藥，200 mg/kg）組，吡咯烷二硫代氨基甲酸鹽（PDTC，NF-κB抑制劑/抗氧化劑，200 mg/kg）組。ASPs、聯(lián)苯雙酯滴丸組每天ig給藥1次，連續(xù)7 d；對照組、模型組和PDTC組以0.1 mL/10 g生理鹽水ig給藥。于末次ig后，PDTC組ip PDTC，再于0.5 h后，除對照組外均尾iv 20 mg/kg Con A誘導(dǎo)肝損傷。8 h后處死動物取材，試劑盒法檢測血清中天冬氨酸轉(zhuǎn)氨酶（AST）、丙氨酸轉(zhuǎn)氨酶（ALT），血清、脾臟組織中白細(xì)胞介素-2（IL-2）、白細(xì)胞介素-4（IL-4）、干擾素-γ（IFN-γ）的蛋白含量；實(shí)時熒光定量PCR（qRT-PCR）法檢測脾臟組織中IL-2、IL-4、IFN-γ mRNA表達(dá)。結(jié)果 與模型組比較，ASPs顯著降低血清中AST、ALT，IL-2、IL-4、INF-γ血清、脾臟組織中的蛋白含量及脾臟組織中mRNA的表達(dá)（P<0.05、0.01），且均呈劑量相關(guān)性。結(jié)論 ASPs降低轉(zhuǎn)氨酶活性、減少炎性細(xì)胞因子的分泌和表達(dá)，對免疫性肝損傷有一定的保護(hù)作用。

Objective To discuss the protective effect of Acanthopanax senticosus polysaccharides (ASPs) by using of Con A causing immunological liver injury model. Method Seventy mice were randomly divided into seven groups, named as control group, model group (Con A), low-, medium-, and high- dose (36.25, 72.5, and 145 mg/kg) groups of ASPs, Bifendate (20 mg/kg, positive drug) group and PDTC (NF-kappa B inhibitor/anti-oxidant, 200 mg/kg) group. The treatment group and positive control group were ig given ASPs and bifendate once daily for 7 d. Control group, model group and PDTC group were ig given normal saline once daily for 7 d. And at the last time, the PDTC group were ip injected PDTC (200 mg/kg). After 0.5 h, immunological liver injury was induced by Con A (20 mg/kg) by injecting via the tail vein in mice. Control group injected the same dose of normal saline. The mice were killed in 8 h later as well as taken tissues and blood using for detecting indexes. Serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were detected by kits. The contents of interleukin-2 (IL-2), interleukin-4 (IL-4), and interferon gamma (IFN-γ) in serum and spleen tissues were detected. Real time fluorescence quantitative PCR (qRT-PCR) was used to detect IL-2, IL-4, and IFN-γ expression in spleen tissues. Results Compared with model group, ASPs not only could reduce the activity of ALT and AST, but also decrease the content of IL-2, IL-4, INF-γ, and RNA expression siginificanly (P<0.05 and 0.01). Conclusion Results showed that ASPs not only can decrease transaminases activity, but also reduce the secretion and RNA expression of inflammatory cytokines. Consequenly, ASPs has a protective effect on immunological liver injury.

黑龍江省博士后資助項(xiàng)目（LBH-Z16196）；黑龍江省應(yīng)用技術(shù)研究與開發(fā)計(jì)劃項(xiàng)目（2013G1031）