目的 研究阿膠對免疫低下小鼠免疫功能的影響。方法 小鼠ip環(huán)磷酰胺或氫化可的松建立免疫低下模型，高、中、低（3.00、1.50、0.75 g/kg）劑量阿膠ig給予小鼠14 d后，測定血清溶血素水平，觀察阿膠對體液免疫的影響；進(jìn)行二硝基氯苯誘導(dǎo)的小鼠耳廓腫脹實(shí)驗(yàn)，檢測小鼠耳廓腫脹度，觀察阿膠對細(xì)胞免疫的影響；流式細(xì)胞術(shù)檢測阿膠對淋巴細(xì)胞亞群的影響；進(jìn)行碳廓清實(shí)驗(yàn)，檢測廓清指數(shù)K和吞噬指數(shù)α，觀察阿膠對非特異性免疫的影響。結(jié)果 與模型組比較，阿膠3.0、1.5、0.75 g/kg均可顯著提高免疫功能低下小鼠血清溶血素含量（P<0.001）；3.0、1.5 g/kg能明顯提高小鼠耳廓腫脹度（P<0.01、0.001）；3 g/kg能顯著提高小鼠CD3⁺（T淋巴細(xì)胞）、CD3⁺CD4⁺（輔助性和遲發(fā)超敏性T細(xì)胞）占淋巴細(xì)胞百分比（P<0.05）；阿膠對小鼠的碳粒廓清指數(shù)K和吞噬指數(shù)α無顯著影響。結(jié)論 阿膠能顯著提高免疫低下小鼠的體液免疫和細(xì)胞免疫，提高CD3⁺、CD3⁺CD4⁺陽性細(xì)胞比例，對非特異性免疫無明顯影響。

Objective To study the effect of Asini Corii Colla (ACC) on immune function in hypoimmune mice. Methods ACC of 3.00, 1.50 and 0.75 g/kg was given to hypoimmune model mice induced by Cyclophosphamide or Hydrocortisone for 14 d. The serum hemolysin content were measured to determine the effect of ACC on humoral immunity. The mouse auricle swelling test induced by two nitrochlorobenzene was carried out to detect the swelling of the auricle in mice, and the effect of ACC on the cell immunity was observed. Flow cytometry was used to detect the effect of ACC on lymphocyte subsets. The carbon clearance experiment was carried out to detect the clearance index K and phagocytic index α, and the effect of ACC on non-specific immunity was observed. Results Compared with model group, ACC could significantly promote the serum hemolysin content (P<0.001) at dose of 3.00, 1.50, and 0.75 g/kg, significantly increase the swelling of auricle in mice (P<0.01, 0.001) at doses of 3.00 and 1.50 g/kg, and significantly increase percentage of CD3⁺ (T lymphocytes), CD3⁺CD4⁺ (helper T cells and delayed hypersensitive T cells) at dose of 3.00 g/kg. There was no significant effect on the phagocytic index K and the clearing index α. Conclusion ACC can stimulate specific immune function and promote the proportion of CD3⁺, CD3⁺CD4⁺ positive cells, it has no obvious effect on non-specific immunity.

國家科技重大專項(xiàng)（2015ZX09501004）；天津市科技計(jì)劃項(xiàng)目（16PTGCCX00090）；山東省重點(diǎn)研發(fā)計(jì)劃項(xiàng)目（2016GGH4514）；國家中藥標(biāo)準(zhǔn)化項(xiàng)目（ZYBZH-Y-SD-31）