目的 探討復(fù)方HD對環(huán)磷酰胺誘導(dǎo)的免疫抑制小鼠的免疫增強作用。方法 ICR小鼠分別以蒸餾水（對照組、模型組）、香菇多糖（200 mg/kg）、復(fù)方HD（5、10、20 g/kg）每天1次ig給藥，連續(xù)10 d；除對照組外，在第4~6天ip環(huán)磷酰胺（40 mg/kg）制備免疫抑制小鼠模型（對照組除外）；每天給藥前精確稱量并記錄小鼠體質(zhì)量。于最后一次給藥24 h后，測定小鼠脾指數(shù)、胸腺指數(shù)；流式細(xì)胞術(shù)進(jìn)行全血中T細(xì)胞亞群分析；ELISA法檢測血漿中IFN-γ和IL-4水平。結(jié)果 與模型組比較，復(fù)方HD低、中、高劑量組顯著提高小鼠的胸腺指數(shù)，CD3⁺、CD3⁺CD4⁺細(xì)胞數(shù)以及CD4⁺/CD8⁺比值（P<0.05），顯著恢復(fù)血漿中IFN-γ水平和IFN-γ/IL-4比值（P<0.05），糾正了Th1/Th2偏移，并呈現(xiàn)出一定的濃度相關(guān)性。結(jié)論 復(fù)方HD能夠促進(jìn)環(huán)磷酰胺誘導(dǎo)的免疫抑制小鼠免疫功能的恢復(fù)，證明其在預(yù)防及治療免疫功能低下方面具有一定的療效。

Objective To systematically elucidate the in vivo immune-enhancement effects of Fufang HD in immunosuppressed mice induced by cyclophosphamide treatment. Methods ICR mice received gastric administration of physiological saline (control group), lentinan (200 mg/kg), Fufang HD low-, medium-, and high-dose (5, 10, and 20 g/kg) once daily for 10 consecutive days, respectively, accompanied by intraperitoneal injection of cyclophosphamide (40 mg/kg) on days 4-6. Accurately weighing and recording the body mass of mice every day. After 24 h of the last administration, spleen index and thymus index of mice were detected. Flow cytometry was used to analyze T cell subsets in whole blood, and the levels of IFN-and IL-4 in plasma were detected by ELISA. Results Compared with model group, low-, medium-, and high-dose Fufang HD treatment accelerated recovery dose-dependently of thymus index, lymphocytic percentages of CD3⁺, CD4⁺, and CD4⁺/CD8⁺ as well as restored the level of serum INF-γ (P<0.05) and corrected Th1/Th2 deviation. Conclusions The results demonstrate that treatment with Fufang HD results in accelerating recovery of immunosuppression in Cy-treated mice for the first time, which is competent in taking into consideration for both precautions and remedy.

遼寧省科學(xué)技術(shù)計劃項目（2014226033）；中國醫(yī)科大學(xué)校內(nèi)課題基金項目（XZR20160007）；中國醫(yī)科大學(xué)附屬第一醫(yī)院院內(nèi)基金項目（FSFH201717）；中國醫(yī)科大學(xué)附屬第一醫(yī)院藥學(xué)部科室基金