目的 探討炎調(diào)方對(duì)膿毒癥急性肺損傷（ALI）大鼠肺組織熱休克蛋白70（HSP70）mRNA和p38絲裂原活化蛋白激酶（p38 MAPK）的調(diào)控作用。方法 將清潔級(jí)健康雄性SD大鼠隨機(jī)分為假手術(shù)組、模型組、炎調(diào)方（生藥量9.9 g/kg）組、谷氨酰胺組、槲皮素組，采用盲腸結(jié)扎穿孔術(shù)法（CLP）建立膿毒癥ALI模型。觀察大鼠一般狀況，各組分別于術(shù)后4、6、8、10、12、18、24 h采集肺組織標(biāo)本，HE染色觀察肺組織病理學(xué)改變；實(shí)時(shí)熒光定量PCR法檢測(cè)肺組織HSP70 mRNA的表達(dá)水平；Western Blotting法檢測(cè)肺組織p38 MAPK的蛋白表達(dá)水平。結(jié)果 炎調(diào)方組、谷氨酰胺組大鼠與模型組比較，術(shù)后不同時(shí)間點(diǎn)的精神狀態(tài)、活動(dòng)量等明顯好轉(zhuǎn)，可見少量稀便；肺組織損傷程度明顯減輕，腔內(nèi)出血較少，肺實(shí)變較輕。炎調(diào)方組不同時(shí)間點(diǎn)的肺組織HSP70 mRNA表達(dá)水平顯著高于假手術(shù)組、模型組（除24 h時(shí)間點(diǎn)外）、槲皮素組（P<0.01），在8、12、18、24 h時(shí)間點(diǎn)顯著低于谷氨酰胺組（P<0.01）；炎調(diào)方組不同時(shí)間點(diǎn)的肺組織p38 MAPK的蛋白表達(dá)量顯著低于模型組、槲皮素組（P<0.01），在4、6、10 h時(shí)間點(diǎn)明顯低于谷氨酰胺組（P<0.05、0.01）。結(jié)論 炎調(diào)方可通過上調(diào)肺組織HSP70 mRNA的表達(dá)，下調(diào)肺組織p38 MAPK的蛋白表達(dá)，改善肺組織損傷，對(duì)膿毒癥ALI發(fā)揮保護(hù)作用。

Objective To investigate the regulation effect of Yantiao Prescription (YP) on the expression of heat shock protein 70 mRNA (HSP70) and p38 mitogen activated protein kinase (p38 MAPK) in lung tissue of rats with acute lung injury induced by sepsis. Methods Healthy male Sprague Dawley rats were randomly divided into sham group, model group, YP group, glutamine group, and quercetin group. Acute lung injury model induced by sepsis was established in rats by cecal ligation and puncture (CLP). At the corresponding time points, ten rats of each group were sacrificed and lung tissue samples were collected. The pathological changes of lung tissue were observed by HE staining. The expression of HSP70 mRNA in lung tissue was detected by real-time PCR. The expression of p38 MAPK in lung tissue was detected by Western Blotting. Results Compared with model group, the mental state and activity of the rats at different time points were obviously improved after the operation, and a small amount of loose stools was observed. The injury degree of lung tissue was obviously reduced, the hemorrhage in the cavity was less, and the pulmonary consolidation was lighter. The expression levels of HSP 70 mRNA in YP group were significantly higher than sham group, model group (except the 24 h time point), and quercetin group (P<0.01), lower than that of glutamine group in 8, 12, 18, and 24 h time point (P<0.01). The relative expression of p38 MAPK in lung tissue of rats in YP group was lower than that in model and quercetin group (P<0.01), and were lower than that of glutamine at the time points of 4, 6, and 10 h (P<0.05). Conclusion YP can improve the expression of lung tissue HSP70 mRNA, reduce the expression of p38 MAPK in lung tissue, improve lung histopathological changes, and plays a protective role against acute lung injury induced by sepsis.

國家自然科學(xué)基金資助項(xiàng)目（81303105）