目的 探究異氟醚對高級別腦膠質(zhì)瘤細(xì)胞MYC基因表達(dá)及細(xì)胞增殖轉(zhuǎn)移能力的影響。方法 分別采用含2%異氟醚的氣體環(huán)境及常規(guī)氣體環(huán)境（5% CO₂）培養(yǎng)對數(shù)生長期的人腦膠質(zhì)瘤細(xì)胞系SHG-44 6 h，作為異氟醚組及對照組；CCK-8實驗檢測兩組細(xì)胞氣體暴露完成后0、24、48、72 h相對增殖能力；Transwell侵襲及遷移實驗檢測兩組細(xì)胞侵襲及遷移能力；Western Blotting檢測兩組細(xì)胞C-Myc及N-Myc的蛋白表達(dá)。結(jié)果 異氟醚組細(xì)胞于暴露完成后48、72 h時增殖能力較對照組細(xì)胞顯著下調(diào)，差異具有統(tǒng)計學(xué)意義（P<0.01）；異氟醚組穿透基質(zhì)膠的侵襲細(xì)胞數(shù)、穿過微孔的遷移細(xì)胞數(shù)均較對照組顯著減少，差異均具有統(tǒng)計學(xué)意義（P<0.01、0.001）；異氟醚組C-Myc及N-Myc的表達(dá)較對照組均顯著下調(diào)，差異具有統(tǒng)計學(xué)意義（P<0.01）。結(jié)論 異氟醚可通過下調(diào)C-Myc及N-Myc基因的表達(dá)，抑制人高級別腦膠質(zhì)瘤細(xì)胞的增殖轉(zhuǎn)移。

Objective To investigate the effect of isoflurane on the expression of MYC gene and cell proliferation and metastasis in high grade glioma cells. Methods The gas environment containing 2% isoflurane and conventional gas environment (5% CO₂)for the cultivation of human glioma cell line SHG-44 6 h in logarithmic growth phase, as the isoflurane group and the control group. CCK-8 assay detected the relative proliferation ability of two groups of cells in 0, 24, 48, and 72 h. Transwell invasion and migration assay detected invasion and migration ability of two groups. Western blotting assay detected the expression of C-Myc and N-Myc in two groups. Results The proliferation ability of isoflurane group was decreased compared to control group in 48 and 72 h, and the difference was statistically significant (P<0.01). The number of invasion cells through Matrigel in isoflurane group was significantly reduced compared to control group, and the number of migrating cells through chamber in isoflurane group was significantly reduced compared to the control group cells, the difference was statistically significant (P<0.01 and 0.001). The expression of C-Myc and N-Myc in isoflurane group were significantly reduced compared to the control group, the difference was statistically significant (P<0.01). Conclusion Isoflurane can inhibit the proliferation and metastasis of human high grade glioma cells by down-regulating the expression of C-Myc and N-Myc genes, and has potential clinical value.