目的 探究七氟醚對(duì)卵巢癌細(xì)胞SKOV3干細(xì)胞特性及增殖、侵襲遷移能力的影響。方法 常規(guī)培養(yǎng)人卵巢癌細(xì)胞SKOV3于培養(yǎng)瓶中，分為七氟醚組及對(duì)照組，七氟醚組以5% CO₂和1%七氟醚培養(yǎng)24 h，對(duì)照組僅以5% CO₂培養(yǎng)24 h，MTT法檢測(cè)兩組細(xì)胞增殖能力的變化，Transwell侵襲及遷移實(shí)驗(yàn)檢測(cè)細(xì)胞侵襲及遷移能力，Western blotting檢測(cè)兩組細(xì)胞干細(xì)胞特性相關(guān)分子（Oct4及Sox2）及上皮間充質(zhì)轉(zhuǎn)化（EMT）相關(guān)分子（E-cadherin、Vimentin）的表達(dá)差異。結(jié)果 七氟醚組細(xì)胞72、96 h時(shí)間點(diǎn)增殖能力顯著低于對(duì)照組，差異具有統(tǒng)計(jì)學(xué)意義（P<0.05）。七氟醚組穿透Matrigel基質(zhì)膠的細(xì)胞數(shù)、遷出Transwell上小室的細(xì)胞數(shù)均明顯少于對(duì)照組，差異具有統(tǒng)計(jì)學(xué)意義（P<0.05）。七氟醚組Oct4、Sox2、Vimentin表達(dá)均顯著低于對(duì)照組，E-cadherin表達(dá)顯著高于對(duì)照組，差異具有統(tǒng)計(jì)學(xué)意義（P<0.05）。結(jié)論 七氟醚可通過(guò)減輕卵巢癌細(xì)胞EMT進(jìn)程及干細(xì)胞特性，抑制細(xì)胞增殖及侵襲遷移能力。

Objective To investigate the effects of sevoflurane on the characteristics of stem cells, proliferation, invasion and migration ability of ovarian cancer cell SKOV3. Methods Human ovarian cancer cells SKOV3 in culture flask were divided into sevoflurane and control group. The sevoflurane group cell was cultured under environment of 5%CO₂ and 1% sevoflurane for 24 h, and the control group cell was cultured under environment of 5%CO₂ for 24 h. MTT method was used to detect the ability of cell proliferation of two groups of cell. Transwell invasion and migration assay were used to detect the ability of cell invasion and migration of two groups of cell. Western blotting was used to detect the expression of stem cell related molecular (Oct4 and Sox2) and epithelial mesenchymal transition (EMT) related molecules (E-cadherin, Vimentin). Results The proliferation ability of the sevoflurane group was significantly lower than that of the control group at 72 and 96 h, and the difference was statistically significant (P<0.05). The number of sevoflurane cells through the matrigel and transwell chamber were less than control group, the difference was statistically significant (P<0.05). The expression of molecular stem cell related Oct4 and Sox2 in sevoflurane group were lower than that in control group, the difference was statistically significant (P<0.05). The expression of molecular EMT related E-cadherin and Vimentin were lower than that in control group, the difference was statistically significant (P<0.05). Conclusion Sevoflurane can inhibit the ability of proliferation, invasion and migration in ovarian cancer cells by reducing the EMT process and stem cell characteristics.