目的 建立芪柏顆粒的UPLC特征圖譜，為評價不同批次成品的質(zhì)量提供參考。方法 采用ACQUITY UPLC HSS T3 C₁₈（100 mm×2.1 mm，1.8 μm）作為色譜柱，以乙腈-0.3%甲酸水為流動相，梯度洗脫，柱溫28℃，波長254 nm，體積流量0.4 mL/min。結(jié)果 建立芪柏顆粒的UPLC特征圖譜，12批不同批次成品相似度均>0.92，共標定46個共有峰，篩選其中25個共有峰與單味藥材進行比對并歸屬，其中4個共有峰來源牡丹皮，4個共有峰來源甘草，2個共有峰來源炙黃芪，2個共有峰來源墨旱蓮，1個共有峰來源卷柏，12個共有峰未進行歸屬。結(jié)論 成功建立芪柏顆粒的UPLC特征圖譜分析方法，其精密度、重復性和穩(wěn)定性良好，可為芪柏顆粒質(zhì)量標準提升作參考。

Objective To establish a UPLC characteristic chromatogram of Qibai Granule for quality control. Methods The ACQUITY UPLC HSS T3 C₁₈ column (100 mm×2.1 mm, 1.8 μm) was used with a mobile phase comprised of acetonitrile-0.3% formic acid for gradient elution. The column temperature was 28℃. The detection wavelength was 254 nm. The flow rate was 0.4 mL/min. Results The UPLC characteristic chromatogram of Qibai Granule was established. The similarities of 12 batches of Qibai Granule were above 0.92. A total of 46 common peaks were found, where 25 common peaks were filtered for belonging to seven medicinal herbs. Among them, four mutual peaks were from Moutan cortex, four mutual peaks were from Glycyrrhizae radix et rhizoma, two common peaks came from Astragali radix praeparata cum melle, two common peaks came from Ecliptae herba, one common peak came from Selaginellae herba, and twelve were unknown sources. Conclusion The method is accurate, repeatable and stable, which can provide the reference for the study of quality control.