目的 觀察銀杏內(nèi)酯注射液、銀杏內(nèi)酯ABC以及銀杏葉提取物注射液（EGb761）對(duì)家兔血小板聚集功能、超微結(jié)構(gòu)及PF-4和β-TG含量的影響比較。方法 將日本大耳兔分為對(duì)照（生理鹽水）組、EGb761（1.02 mL/kg）組、銀杏內(nèi)酯ABC （5.13 mg/kg）組、銀杏內(nèi)酯注射液（1.02 mL/kg，以萜內(nèi)酯計(jì)5.1 mg/kg）組、白果內(nèi)酯（5.13 mg/kg）組，分別iv相應(yīng)藥物1周。給藥結(jié)束后，家兔心臟取血，觀察PAF誘導(dǎo)下血小板聚集率；電鏡觀察血小板超微結(jié)構(gòu)；試劑盒法觀察PAF誘導(dǎo)下血清中血小板因子-4（PF-4）和β-血小板球蛋白（β-TG）含量。結(jié)果 與對(duì)照組比較，EGb761組、銀杏內(nèi)酯ABC組、銀杏內(nèi)酯注射液組的血小板聚集率均顯著降低（P<0.05、0.01），白果內(nèi)酯組無(wú)顯著變化；血小板聚集抑制率：銀杏內(nèi)酯注射液（43.76%） > 銀杏內(nèi)酯ABC （35.3%） > EGb761（26.52%） > 白果內(nèi)酯（5.48%）。與對(duì)照組比較，EGb761、銀杏內(nèi)酯ABC、銀杏內(nèi)酯注射液均能減少聚集型血小板數(shù)量，使樹(shù)突型血小板突起變少變短。與對(duì)照組比較，銀杏內(nèi)酯注射液和銀杏內(nèi)酯ABC均使PF-4和β-TG表達(dá)顯著降低（P<0.01），EGb761僅顯著降低β-TG表達(dá)（P<0.05）。結(jié)論 銀杏內(nèi)酯注射液通過(guò)PAF途徑發(fā)揮抗血小板聚集作用，其作用強(qiáng)于銀杏內(nèi)酯ABC，可能是白果內(nèi)酯發(fā)揮了協(xié)同增效作用。

Objective To observe the effects of Ginkgolide Injection, ginkgolide ABC and ginkgo biloba extract (EGb761) on platelet aggregation function, ultrastructure, and contents of PF-4 and β-TG in rabbits.Methods Rabbits were divided into control (physiological saline) group, EGb761 (1.02 mL/kg) group, ginkgolide ABC (5.13 mg/kg) group, Ginkgolide Injection (1.02 mL/kg, as terpene lactones 5.1 mg/kg) group, and bilobalide (5.13 mg/kg) group, respectively. The corresponding drugs were iv injected for one week respectively. After administration, the blood was taken in the heart of rabbits. The platelet aggregation rate induced by platelet activating factor (PAF) was observed, and the ultrastructure of the platelets was observed by electron microscope, and the content of platelet factor-4 (PF-4) and beta thromboglobulin (β-TG) in serum induced by PAF was observed by the kit method.Results Compared with control group, the platelet aggregation rates in group EGb761, ginkgolide ABC, and Ginkgolide Injection were significantly decreased (P < 0.05 and 0.01), and no significant changes were found in the group of bilobalide. The inhibition rate of platelet aggregation was Ginkgolide Injection (43.76%) > ginkgolide ABC (35.3%) > EGb761 (26.52%) > bilobalide (5.48%). Compared with control group, EGb761, ginkgolide ABC and Ginkgolide Injection could reduce the number of aggregated platelets and make dendritic protrusions shorter; Ginkgolide Injection and ginkgolide ABC significantly decreased the expression of PF-4 and β-TG (P < 0.01), and EGb761 only decreased the expression of β-TG (P < 0.05).Conclusion Ginkgolide injection plays an anti-platelet aggregation effect through PAF pathway with better effect than ginkgolide ABC, which may be that ginkgolide plays a synergistic effect.