腫瘤是一類(lèi)與細(xì)胞周期密切相關(guān)的疾病。流式細(xì)胞術(shù)對(duì)腫瘤細(xì)胞周期的分析可以觀察到腫瘤細(xì)胞倍體的變化，有助于腫瘤的早期發(fā)現(xiàn)和治療。區(qū)分腫瘤細(xì)胞所處于的細(xì)胞周期，明確地了解每個(gè)時(shí)相所占細(xì)胞周期的比例，對(duì)于腫瘤藥物的研發(fā)具有重要意義。該文著重對(duì)G₀期和G₁期，G₂期和M期，以及S期細(xì)胞的測(cè)定方法進(jìn)行歸納、總結(jié)，并對(duì)這些方法的優(yōu)缺點(diǎn)及適用條件進(jìn)行闡述和總結(jié)，為腫瘤藥物的研發(fā)及基礎(chǔ)研究等方面的科研工作提供參考。

Tumor is a kind of disease that is closely related to cell cycle. The analysis of tumor cell cycle by flow cytometry can observe the change of tumor cell ploidy, which is helpful for early detection and treatment of tumor. It is of great significance to distinguish the cell cycle of tumor cells and to understand the proportion of cell cycle in each phase. In this paper, the determination methods on the G₀ and G₁ phase, the G₂ and M, and S phase are summarized, and at the same time we expounds the advantages and disadvantages of these methods and the suitable conditions., which provide an reference for cancer drug research and pharmacological research.

哈爾濱商業(yè)大學(xué)研究生創(chuàng)新科研資金項(xiàng)目（YJSCX2017-456HSD）；黑龍江省衛(wèi)生計(jì)生委科研課題（2017-132）；哈爾濱市科技創(chuàng)新人才研究專(zhuān)項(xiàng)資金項(xiàng)目（2017RAXXJ085）；黑龍江省教育廳科學(xué)技術(shù)研究項(xiàng)目（12541571）；黑龍江省高等學(xué)校創(chuàng)新團(tuán)隊(duì)建設(shè)計(jì)劃項(xiàng)目（2014TD009）；哈爾濱商業(yè)大學(xué)科研團(tuán)隊(duì)計(jì)劃項(xiàng)目（2016TD002）；黑龍江省級(jí)領(lǐng)軍人才梯隊(duì)后備帶頭人資助項(xiàng)目