目的 探討抗骨髓炎片對家兔慢性骨髓炎的作用。方法 采用右脛骨骨髓腔內(nèi)注射金黃色葡萄球菌的方法建立家兔慢性骨髓炎模型，隨機分為4組：抗骨髓炎片高、低劑量（480、240 mg/kg）組，慶大霉素（60 mg/kg）組，模型組（等體積0.5% CMC-Na）；另取家兔向骨髓腔內(nèi)注入生理鹽水，作為假手術(shù)組（等體積0.5% CMC-Na），術(shù)后當(dāng)天開始ig給藥，每天1次，連續(xù)6周。術(shù)后第6周對右脛骨行X-線檢查并做Laurence評分；術(shù)后第2、4、6周采血，血細胞分析儀進行白細胞計數(shù)，ELISA法測定血清溶菌酶、腫瘤壞死因子-α（TNF-α）和白細胞介素-6（IL-6）含量。結(jié)果 模型組家兔均有典型骨髓炎表現(xiàn)，抗骨髓炎片高、低劑量對骨髓炎癥狀有顯著緩解，Laurence評分與模型組比較顯著降低（P<0.01）；與模型組比較，抗骨髓炎片高、低劑量組的白細胞計數(shù)、血清TNF-α和IL-6含量在術(shù)后2、4、6周均明顯降低（P<0.01），高劑量組血清溶菌酶含量在術(shù)后2、4、6周均明顯升高（P<0.01），低劑量組血清溶菌酶含量在第4、6周亦明顯升高（P<0.05）。結(jié)論 抗骨髓炎片對家兔慢性骨髓炎發(fā)揮顯著改善作用，可能與抑制致病菌、提高機體免疫力、下調(diào)炎性因子等途徑有關(guān)。

Objective To investigate the effects of Kanggu Suiyan Tablet (KST) against osteomyelitis in rabbits. Methods Osteomyelitis model of rabbits were prepared by intra-shinbone marrow injection of Staphylococcus aureus. Model rabbits were divided at random into four groups, i.e., model (equal volume 0.5% CMC-Na), gentamicin (60 mg/kg), KT high and low dose groups (480 and 240 mg/kg, respectively). The rabbits were injected with saline into the bone marrow cavity as sham group (equal volume 0.5% CMC-Na). Drugs were ig administrated after the operation once daily for six weeks. On the 6th week of operation, right shinbone X-ray in rabbits were examined and laurence scores were calculated. Leucocyte counts were determined by blood cell analyzer, lysozyme, TNF-α, and IL-6 levels in sera were determined by ELISA on the 2nd, 4^th, and 6th weeks, respectively. Results Typical symptoms of osteomyelitis were identified in model group. Laurence scores in KT groups (480 and 240 mg/kg) were significantly reduced compared with that of model group (P<0.01). Comparing with model group, leucocyte count in KT groups were evidently increased on the 2nd, 4th, and 6th week, respectively (P<0.01). Lysozyme levels were evidently increased on the 2nd, 4th, and 6th week, respectively, in high dose group (P<0.01) and 4th and 6th week, respectively, in low dose group (P<0.05). TNF-α and IL-6 levels in sera of KT groups were evidently decreased on the 2nd, 4th, and 6th week, respectively (P<0.05 or 0.01). Conclusion The protective effect of KT on osteomyelitis seems to be associated with inhibition of pathogenic bacteria, improvement of immune function and downregulation of inflammatory cytokines, etc.