目的 對(duì)白花敗醬草中總黃酮的提取工藝和抗腹瀉活性進(jìn)行初步研究。方法 以蘆丁為對(duì)照品，以單位質(zhì)量生藥中總黃酮含量為指標(biāo)，采用硝酸鋁顯色比色-紫外分光光度法，通過(guò)單因素考察和響應(yīng)面設(shè)計(jì)優(yōu)化有機(jī)溶劑法提取白花敗醬草莖總黃酮的工藝，分別考察乙醇濃度、料液比、提取時(shí)間和提取溫度對(duì)白花敗醬草總黃酮（PTF）提取效果的影響。通過(guò)小鼠小腸酚紅推進(jìn)實(shí)驗(yàn)和大鼠離體腸平滑肌實(shí)驗(yàn)評(píng)估白花敗醬草總黃酮在體內(nèi)、外對(duì)腹瀉模型中腸動(dòng)力的影響。結(jié)果 經(jīng)優(yōu)化，在溶劑為60%乙醇、料液比1：8 g/mL、85℃提取2.5 h的條件下，白花敗醬草莖的總黃酮含量可達(dá)25 mg/g以上。白花敗醬草總黃酮可以顯著降低乙酰膽堿誘發(fā)的收縮亢進(jìn)的大鼠離體腸平滑肌收縮張力，且對(duì)不同腸段的作用強(qiáng)度順序?yàn)椋嚎漳c > 十二指腸 > 回腸 > 結(jié)腸。與模型組比較，白花敗醬草總黃酮能夠明顯抑制酚紅在蓖麻油致腹瀉小鼠小腸中的推進(jìn)進(jìn)程（P<0.05、0.01）。結(jié)論 有機(jī)溶劑法提取白花敗醬草莖中總黃酮的工藝通過(guò)響應(yīng)曲面分析得到優(yōu)化，白花敗醬草總黃酮可以抑制腸蠕動(dòng)，具有潛在的抗腹瀉活性。

Objective To optimize the extracting conditions of total flavonoids from Patrinia villosa stem and to preliminarily investigate the antidiarrheal activity of total flavonoids from P. villosa. Methods UV spectrophotometry was used to determine the content of total flavonoids by taking rutin as the standard substance, aluminum nitrate as the chromogenic agent and taking the content of total flavonoids in per gram of crude drug as the index. The extracting technologies were evaluated through signal factor study and response-surface-design experiments. The in vitro and in vivo effects of total flavonoids of P. villosa on intestinal motility in the diarrheal model were evaluated by isolated intestine test and testing the propelling of phenol red in small intestine of mice. Results The optimum extracting conditions of total flavonoids from P. villosa stem includes the extracting solvent of 60% ethanol, ratio of material to liquid at 1:8 g/mL, extracting time of 2.5 h and the temperature at 85℃, under which condition the content of total flavonoids from P. villosa stem could reach over 25 mg/g. The total flavonoids of P. villosa can significantly reduce the contractile tension of isolated acetylcholine-induced hyperactive rat intestinal smooth muscles, and the order of effect on different intestinal segments was:jejunum > duodenum > ileum > colon. The total flavonoids of P. villosa significantly inhibit the progress of phenol red in the small intestine of castor oil-loaded diarrhea mice (P<0.05, 0.01). Conclusion The organic solvent-extracting conditions of total flavonoids from the stems of P. villosa were optimized by response surface analysis. The total flavonoids of P. villosa inhibit intestinal peristalsis and were verified to possess the potential antidiarrheal activity.

R962.2

國(guó)家自然科學(xué)基金項(xiàng)目（81673693）；現(xiàn)代中藥創(chuàng)新中心創(chuàng)新能力建設(shè)項(xiàng)目