目的 觀察抗骨髓炎片（KST）對家兔慢性骨髓炎（CO）的改善作用。方法 將金黃色葡萄球菌注入家兔右脛骨骨髓腔內(nèi)建立CO模型，將CO家兔分為模型組、慶大霉素（60 mg/kg）組及KST高、低劑量（480、240 mg/kg）組，另設(shè)假手術(shù)組，ig給藥。術(shù)后第2、4、6周對右脛骨行X-線檢查并做診斷分型，采血進(jìn)行白細(xì)胞計(jì)數(shù)，取血清檢測C-反應(yīng)蛋白（CRP）含量，術(shù)后第6周取右脛骨檢測核因子κB（NF-κB）的陽性表達(dá)。結(jié)果 KST高、低劑量組術(shù)后第2、4、6周的X-片診斷分型與模型組比較均顯著改善，有統(tǒng)計(jì)學(xué)意義（P<0.05、0.01）；與模型組比較，KST高、低劑量組的白細(xì)胞計(jì)數(shù)、血清CRP含量、脛骨組織NF-κB表達(dá)在第2、4、6周均顯著降低（P<0.05、0.01）。結(jié)論 KST對CO家兔具有保護(hù)作用，其機(jī)制可能與抑制致病菌、緩解炎癥反應(yīng)、下調(diào)炎性反應(yīng)調(diào)控因子NF-κB等環(huán)節(jié)有關(guān)。

Objective To observe the protective effect of Kanggusuiyan Tablet (KST) against rabbits with chronic osteomyelitis (CO). Methods CO model of rabbit was achieved by intra-shinbone marrow injection of Staphylococcus aureus. CO rabbits were divided randomly into five groups, including sham group, model group, KST high and low dose (480 and 240 mg/kg, respectively) group and gentamicin (60 mg/kg) group. Drugs were administered orally daily for six weeks. The X-ray of right shinbone in rabbits were evaluated and graded, leucocyte count and level of C-reactive protein (CRP) in serum were examined on the 2nd, 4th and 6th week post-injection, respectively. At the end of the experiment, all the rabbits were sacrificed and the expression of NF-κB in right shinbone was determined by immunohistochemistry. Results The grading of X-ray in KST groups (480 and 240 mg/kg) were significantly improved compared with that of model group on the 2nd, 4th and 6th week, respectively (P<0.05 or P<0.01). Comparing to those of model group, leucocyte count and sera CRP levels in KST groups were significantly reduced on the 2nd, 4th and 6th week, respectively (P<0.01). KST (480 and 240 mg/kg) significantly decreased the expression of NF-κB in shinbone compared with that of model group (P<0.01). Conclusion The protective effect of KST against CO seems to be associated with inhibition of pathogenic bacteria, improvement of inflammation and downregulation of NF-κB, etc.

R978