目的 研究特定聚合度原花青素為主要成分的葡萄籽提取物在腹瀉治療中的新用途。方法 將葡萄籽進(jìn)行粉碎、粗提、精制并分級(jí)沉淀后得到6個(gè)部位，以大鼠腹瀉模型評(píng)價(jià)葡萄籽原花青素提取物各部位的止瀉活性，并對(duì)有效部位的總原花青素含量、平均聚合度和主要單體組成等特性進(jìn)行分析。結(jié)果 來(lái)源于葡萄籽的多聚原花青素部位F4對(duì)應(yīng)激性腹瀉模型有顯著活性，而其他純化部位和總提物并無(wú)此作用。結(jié)論 F4部位在主要原花青素單體組成和含量上與總原花青素及其他部位相比并無(wú)差異，但在平均聚合度上差異明顯，表明F4部位的止瀉活性與其特定的聚合度密切相關(guān)。

Objective The procyanidins was researched as the main component of grape seed extract in the treatment of diarrhea. Methods Grape seeds were crushed, roughly extracted, refined and then six parts were obtained after fractionation. Subsequently, a diarrhea model was used to study the antidiarrheal effectiveness of all parts of the extract. In addition, the characteristics of the total proanthocyanidin contents of active fractions, the mean degrees of polymerization, and the monomer composition were analyzed. Results The polymeric procyanidins site F4 derived from grape seeds on stress diarrhea model had significant activity, while other parts of the purification and the total extract did not have such effect. Conclusion F4 is nothing special compared with the total proanthocyanidins and other parts on the monomer composition and the content of procyanidins, but in significantly difference on the mean degree of polymerization. The results suggest that F4 shows antidiarrheal activity, and its specific was closely related to the degree of polymerization.

天津科市技計(jì)劃項(xiàng)目（12JCQNJC08700）