目的 觀察復(fù)腎湯（FST）對糖尿病腎?。―N）大鼠足細(xì)胞nephrin和podocin表達(dá)的影響，并探討其可能的作用機制。方法 正常對照組為雄性Wistar大鼠8只，常規(guī)飼養(yǎng)。雄性Wistar大鼠24只，正常飼養(yǎng)1周后大鼠ip鏈脲佐菌素（STZ）和弗氏完全佐劑（CFA）建立糖尿病模型。造模成功后分為模型組、FST大劑量組（L1）、FST小劑量組（L2）。L1、L2組分別給予復(fù)腎湯32、8 g/kg；模型組給予生理鹽水。每天ig給藥1次，給藥體積1 mL/100 g，連續(xù)8周，定期測血糖。實驗結(jié)束后，收集24 h尿液，采集血清，檢測胰島素（INS）、肌酐（SCr）、尿素氮（BUN）、超氧化物歧化酶（SOD）、丙二醛（MDA）、尿蛋白（UP）等生化指標(biāo)；輕取腎臟，光鏡下觀察腎臟組織病理變化；免疫熒光法檢測腎組織nephrin和podocin的表達(dá)，RT-qPCR法檢測腎組織nephrin、podocin的基因表達(dá)。結(jié)果 與模型組比較，L1、L2組的INS、SCr、BUN、MDA、UP明顯降低（P<0.05、0.01），SOD明顯升高（P<0.05），病理學(xué)評分明顯降低（P<0.05），nephrin、podocin的免疫熒光表達(dá)明顯上調(diào)，RT-qPCR基因表達(dá)明顯上調(diào)。結(jié)論 復(fù)腎湯可以有效改善DN大鼠足細(xì)胞nephrin和podocin的表達(dá)，對DN的發(fā)展具有一定的延緩作用。

Objective To observe the effects of Fushentang on nephrin and podocin in podocytes of diabetic nephropathy rats and discuss the possible mechanism. Methods The normal control group was eight male Wistar rats with conventional feeding. A total of 24 male Wistar rats were treated with intraperitoneal injection of STZ + CFA to establish diabetic model after normal feeding for 1 weeks. After successful modeling, the rats were divided into model group, FST supernatant group (L1), FST sediment group (L2). L1, L2 group were treated with FST supernatant and sediment. The model group was given normal saline. Rats were administered ig for once daily with a volume of 1 mL/100 g, and blood glucose was measured regularly for 8 weeks. After the experiment, 24 h urine, the serum were collected, and the biochemical parameters, like INS, SCr, BUN, SOD, MDA, UP were detected. The kidney was removed and the pathological changes were observed under light microscope, the expressions of nephrin and podocin in renal tissues were detected by immunofluorescence, and the gene expressions of nephrin and podocin in renal tissues were detected by RTqPCR.Results Compared with the model group, the INS, SCr, BUN, MDA, UP of L1 and L2 groups were significantly decreased (P<0.05, 0.01), and SOD was increased significantly (P<0.05), the pathology score decreased significantly (P<0.05). Immunofluorescence expressions of nephrin and podocin were significantly up-regulated, and gene expressions of RT-qPCR were significantly up-regulated. Conclusion Fushentang can significantly improve the expression of nephrin and podocin in podocytes of DN rats, and delay the development of diabetic nephropathy.

江蘇省自然科學(xué)基金青年基金項目（BK20180827）