目的 研究有氧運(yùn)動(dòng)對(duì)小鼠主動(dòng)脈自噬相關(guān)因子表達(dá)及自噬活化的影響。方法 將8周齡C57BL/6雄性小鼠40只隨機(jī)分為安靜對(duì)照組（n=20）與有氧運(yùn)動(dòng)組（n=20），其中10只用于Real-Time PCR實(shí)驗(yàn)，10只用于Western-blot實(shí)驗(yàn)，有氧運(yùn)動(dòng)組小鼠進(jìn)行6周的游泳運(yùn)動(dòng)（60 min/d，5 d/周），對(duì)照組小鼠自由活動(dòng)。6周后取小鼠胸腹主動(dòng)脈，采用Real-Time PCR方法檢測(cè)自噬相關(guān)因子ULK1、Beclin1、LC3B mRNA水平；Western-blot方法檢測(cè)主動(dòng)脈LC3B蛋白中LC3B-II/LC3B-Ⅰ比值及p62蛋白水平。結(jié)果 與對(duì)照組相比，有氧運(yùn)動(dòng)組小鼠主動(dòng)脈ULK1、Beclin1、LC3B mRNA水平明顯升高（P<0.05）；有氧運(yùn)動(dòng)組小鼠主動(dòng)脈LC3B-II/LC3B-Ⅰ比值增大（P<0.05），p62蛋白降解增加（P<0.05）。結(jié)論 有氧運(yùn)動(dòng)能夠使主動(dòng)脈ULK1、Beclin1、LC3B自噬相關(guān)因子表達(dá)增強(qiáng)，進(jìn)而增強(qiáng)主動(dòng)脈自噬活性。

Objective To investigate the effect of aerobic exercise on the levels of autophagy-related markers and Autophagy activation in mouse aorta. Methods Twenty C57BL/6 male mice aged 8 weeks were randomly divided into sedentary control group and aerobic exercise group. The aerobic exercise group were subjected to swimming for 6 weeks, 60 min/d, 5 days a week. The Thoracic and abdominal aorta of mice were isolated and detected after 6 weeks. Real-Time PCR was adopted to measure the mRNA levels of autophagy-related markers, including ULK1, Beclin1 and LC3B. Western-blot was applied to measure the expression level of autophagy-related protein including the ratio of LC3B-II/LC3B-Ⅰ and p62. Results Compared with the control group, in the aerobic exercise group, the mRNA levels of ULK1, Beclin1 and LC3B and the ratio of LC3B-II/LC3B-Ⅰ were significantly increased (P<0.05), the protein degradation of p62 was significantly increased (P<0.05). Conclusion Aerobic exercise can increase the expression of ULK1, Beclin1 and LC3B in the aorta, and then enhance the aortic autophagy activity.

山東省高等學(xué)?？萍加?jì)劃項(xiàng)目（J18KB117）；濱州醫(yī)學(xué)院附屬醫(yī)院青年科研基金（2013QNKYJJ02）