目的 利用實(shí)時(shí)細(xì)胞分析（RTCA）Cardio系統(tǒng)，通過體外細(xì)胞實(shí)驗(yàn)及整體動(dòng)物實(shí)驗(yàn)評(píng)價(jià)速效救心丸抗心律失常作用。方法 培養(yǎng)原代大鼠乳鼠心肌細(xì)胞，接種于E-Plate Cardio 96，分析3 600/孔、20 000/孔以及90 000/孔接種濃度心肌細(xì)胞的細(xì)胞指數(shù)（CI）和搏動(dòng)圖形；MTT法檢測速效救心丸（10、20、40、80、160 μg/mL）對(duì)原代心肌細(xì)胞活力的影響；考察速效救心丸（160 μg/mL）對(duì)烏頭堿（8 μmol/L）誘發(fā)心律失常的心肌細(xì)胞搏動(dòng)頻率、搏動(dòng)幅度的影響；舌下iv烏頭堿50 μg/kg制備大鼠心律失常模型，利用八導(dǎo)生理記錄儀觀察速效救心丸（54、270 mg/kg）對(duì)Ⅱ?qū)?lián)心電圖的影響。結(jié)果 20 000/孔為最佳接種濃度，CI值在觀察時(shí)間內(nèi)成對(duì)數(shù)生長，細(xì)胞形成同步搏動(dòng)，波形正常且持續(xù)時(shí)間長；在10～160μg/mL濃度范圍內(nèi)，速效救心丸對(duì)心肌細(xì)胞活力均沒有明顯影響。RTCA Cardio系統(tǒng)檢測結(jié)果顯示，與模型組比較，速效救心丸能顯著降低心肌細(xì)胞搏動(dòng)速率（P<0.01）、顯著增加心肌細(xì)胞的搏動(dòng)幅度（P<0.01）。動(dòng)物實(shí)驗(yàn)發(fā)現(xiàn)，速效救心丸能有效對(duì)抗烏頭堿誘導(dǎo)的心律失常、改善烏頭堿對(duì)實(shí)驗(yàn)動(dòng)物RR間期的抑制作用和對(duì)心率的促進(jìn)作用、降低烏頭堿誘導(dǎo)的室顫發(fā)生率（P<0.01、0.001）。結(jié)論 RTCA Cardio系統(tǒng)能實(shí)現(xiàn)對(duì)心肌細(xì)胞的實(shí)時(shí)監(jiān)測，可以檢測心肌細(xì)胞的搏動(dòng)情況；速效救心丸能有效對(duì)抗烏頭堿誘導(dǎo)的心律失常。

Objective To evaluate the anti-arrhythmia effect of Suxiao Jiuxin Pill in vitro cell experimental and whole animal experiment by using the real-time cell analysis (RTCA) Cardio system, which provided experimental basis for the clinical application of Suxiao Jiuxin Pill. Methods The primary rat cardiac myocytes were cultured and inoculated in e-plate cardio 96. The cell index (CI) and pulsation pattern of cardiac myocytes at 3 600/well, 20 000/well and 90 000/well were analyzed. The effects of Suxiao Jiuxin Pill (10, 20, 40, 80, 160 μg/mL) on the viability of primary cardiac myocytes were detected by MTT method. The effects of Suxiao Jiuxin Pill (160 μg/mL) on aconitine (8 μmol/L) were investigated. The effects of tachycardia frequency and amplitude of cardiac myocytes on arrhythmia; the rat model of arrhythmia was made by sublingual aconitine 50 μg/kg, and the effects of Suxiao Jiuxin Pill (54, 270 mg/kg) on lead II ECG were observed by eight channel physiological recorder. Results In the concentration range of 10- 160 μg/mL, Suxiao Jiuxin Pill had no significant effect on the viability of cardiomyocytes. The results of RTCA cardio system showed that compared with the model group, Suxiao Jiuxin Pill could significantly reduce the beating rate of cardiomyocytes (P < 0.01), and significantly increase the beating range of cardiomyocytes (P < 0.01). Animal experiments showed that Suxiao Jiuxin Pillcan effectively resist aconitine induced arrhythmia, improve the inhibition of aconitine on RR interval and the promotion of heart rate, and reduce the incidence of ventricular fibrillation induced by aconitine (P < 0.01, 0.001). Conclusion RTCA cardio system can realize real-time monitoring of myocardial cells, Suxiao Jiuxin Pill can effectively treat arrhythmiawas induced by Aco.

R965

國家自然科學(xué)基金資助項(xiàng)目（81703710）；天津市留學(xué)回國人員科技活動(dòng)啟動(dòng)項(xiàng)目（2018018）