目的 比較注射用益氣復(fù)脈（凍干）（YQFM）尾iv與ig兩種給藥途徑對(duì)心肌梗死小鼠的改善作用。方法 采用冠狀動(dòng)脈左前降支結(jié)扎誘導(dǎo)小鼠急性心肌梗死模型，飼養(yǎng)1周得到慢性心肌梗死模型。分別通過尾iv與ig給藥兩種途徑給予臨床等效劑量的YQFM（0.867 g/kg），通過測(cè)定不同給藥時(shí)間后血清中乳酸脫氫酶（LDH）和肌酸激酶（CK）水平，比較兩種給藥途徑起效時(shí)間。進(jìn)一步開展心臟超聲、超氧化物歧化酶（SOD）測(cè)定以及心臟HE染色實(shí)驗(yàn)，評(píng)價(jià)兩種給藥途徑在連續(xù)給藥3 d后的藥效學(xué)差異。分別ig給予YQFM 0.867、1.734、3.468 g/kg，尾iv給予YQFM 0.867、1.734 g/kg，評(píng)價(jià)兩種給藥途徑在不同給藥濃度時(shí)的藥效差異。結(jié)果 與模型組比較，急性及慢性心肌梗死小鼠iv給藥3 d或ig給藥7 d后，血清LDH和CK水平顯著降低（P<0.05、0.01、0.001），說明iv給藥組起效時(shí)間早于ig給藥組。連續(xù)給藥3 d后，iv給藥組在改善心臟功能、降低LDH、CK水平，提高SOD活性方面均顯著優(yōu)于ig給藥組；兩種給藥途徑均可改善心肌梗死小鼠心臟病理組織形態(tài)。相同給藥劑量下，iv給藥組較ig給藥組對(duì)急性或慢性心肌梗死小鼠的保護(hù)作用更加顯著。結(jié)論 iv給予YQFM對(duì)于急性或慢性心肌梗死模型小鼠的改善作用較ig給藥途徑更加迅速、高效。

Objective To compared the protective effects of Yiqi Fumai Lyophilized Injection (YQFM) against myocardial infarction mice through intravenous injection and intragastric administration. Methods Acute myocardial infarction model was induced by ligation of the left anterior descending coronary artery, and the model of chronic myocardial infarction was obtained after one week raise. The maximum clinical equivalent dose of YQFM was given by intravenous and intragastric administration respectively. Serum LDH and CK levels were measured at different dosing times, and the onset time of the two routes of administration was compared. The cardiac ultrasound, superoxide dismutase (SOD) and cardiac HE staining were performed to evaluate the pharmacodynamic differences between two routes of administration after 3 days of continuous administration. Evaluate the differences in efficacy between two administration routes at different administration concentrations. Results The acute and chronic myocardial infarction model mice were iv administered for 3 days or 7 days after ig administration. After that, serum LDH and CK levels were significantly decreased (P < 0.05, 0.01 and 0.001), indicating that the onset of iv administration was earlier than the ig administration group. After 3 days of continuous administration, intravenous injection of YQFM was significantly superior to the intragastric administration group in improving cardiac function, decreasing LDH, CK levels, and increasing SOD activity. Both can improve the pathological morphology of model mice heart. Under the same dose, iv administration group was more effective in treatment of acute or chronic myocardial infarction than the ig administration group. Conclusion Compared with ig administration, intravenous injection of YQFM has advantages of earlier onset time and more effective protection in acute or chronic myocardial infarction.

R965

天津市科技計(jì)劃項(xiàng)目（17PTSYJC00090）