目的 探究透骨消痛膠囊對大鼠骨性關(guān)節(jié)炎的抑制作用。方法 將大鼠隨機分成5組：對照組、模型組及透骨消痛膠囊低、中、高劑量（0.93、1.86、3.72 g/kg）組，除對照組外，采用手術(shù)方法構(gòu)建SD大鼠骨性關(guān)節(jié)炎模型，對照和模型組ig給予等量生理鹽水，每天1次，持續(xù)8周。給藥結(jié)束后，大鼠眼眶取血后分離血清，處死大鼠取膝關(guān)節(jié)軟骨。軟骨組織HE染色后用改良Mankin評分細(xì)則進行評分；實時熒光定量PCR（qRT-PCR）和Western blotting檢測軟骨中基質(zhì)金屬蛋白酶（MMP）-2和MMP-13的表達(dá)；ELASA法檢測血清炎性介質(zhì)的表達(dá)。結(jié)果 與模型組比較，透骨消痛膠囊0.93、1.86、3.72 g/kg劑量組HE染色評分均顯著升高（P<0.05），MMP-2、MMP-13 mRNA和蛋白水平均顯著降低（P<0.01）；血清腫瘤壞死因子-α（TNF-α）、血管內(nèi)皮生長因子（VEGF）、白細(xì)胞介素-6（IL-6）和白細(xì)胞介素-1β（IL-1β）水平均顯著降低（P<0.01）。結(jié)論 透骨消痛膠囊可以顯著抑制大鼠骨性關(guān)節(jié)炎，機制與降低MMP-2、MMP-13表達(dá)，降低血清炎癥因子TNF-α、VEGF、IL-6和IL-1-β水平相關(guān)。

Objective To explore the inhibitory Mechanism of Tou Gu Xiao Tong capsule on rat osteoarthritis synovitis. Methods The rats were randomly divided into five groups: control group, model group, low, medium and high dose (0.93, 1.86, 3.72 g/kg) group of Tou Gu Xiao Tong Capsule. Except the control group, SD rats were operated to construct osteoarthritis model. Rats of control group and model group were ig given the same amount of normal saline once a day for 8 weeks. At the end of administration, blood was collected from the orbit of the rats and serum was separated. After HE staining, the cartilage tissue was graded by modified Mankin scoring rules; the expression of matrix metalloproteinase (MMP) -2 and MMP-13 in cartilage was detected by real-time fluorescent quantitative PCR (qRT-PCR) and Western blotting; the expression of serum inflammatory mediators was detected by elasa method. Results Compared with the model group, the HE staining scores of 0.93, 1.86 and 3.72 g/kg groups of tgxtc were significantly higher (P < 0.05), the levels of MMP-2, MMP-13 mRNA and protein were significantly lower (P < 0.01), the levels of TNF - α, VEGF, IL-6 and IL-1 β were significantly lower (P < 0.01). Conclusion TouGu XiaoTong Capsule can significantly inhibit osteoarthritis in rats, the mechanism is related to the decrease of MMP-2, MMP-13 expression, TNF -α, VEGF, IL-6 and IL-1 - β levels.

R965

河南省科技發(fā)展計劃項目（142102310449）