目的 研究金蓮花中酚酸類化合物在大鼠肝微粒體內(nèi)的轉(zhuǎn)化特征。方法 建立體外大鼠肝微粒體轉(zhuǎn)化模型，模擬金蓮花中原金蓮酸、金蓮花苷、藜蘆酸在大鼠肝臟內(nèi)的代謝過程，采用液相色譜質(zhì)譜聯(lián)用（LC-MS）法對(duì)代謝成分進(jìn)行檢測(cè)和分析。結(jié)果 在大鼠肝微粒體的作用下，原金蓮酸轉(zhuǎn)化為1個(gè)產(chǎn)物，分子式為C₁₉H₂₃O₁₀，推測(cè)為原金蓮酸的葡萄糖醛酸化反應(yīng)產(chǎn)物；金蓮花苷轉(zhuǎn)化為2個(gè)產(chǎn)物，分別為C₂₅H₃₆O₁₄和C₁₈H₂₄O₉，推測(cè)分別為增加1糖基和脫甲基生成的轉(zhuǎn)化產(chǎn)物；藜蘆酸轉(zhuǎn)化為1個(gè)產(chǎn)物，分子式為C₈H₈O₄，推測(cè)2種結(jié)構(gòu)，各為脫掉1個(gè)甲基的產(chǎn)物。結(jié)論 金蓮花酚酸類化合物在大鼠肝微粒體中主要發(fā)生脫甲基化、糖苷化、葡萄糖醛酸化反應(yīng)，為明確金蓮花酚酸類成分的體內(nèi)轉(zhuǎn)化規(guī)律和代謝特征提供依據(jù)。

Objective To explore the metabolic characteristics of phenolic acids from the flowers of Trollius chinensis in rat liver microsomes. Methods The rat liver microsomal transformation model was established in vitro to simulate the metabolic process of proglobeflowery acid, trollioside, and veratric acid in rat liver. LC-MS method was employed to detect and analyze the metabolic components. Results Under the action of rat liver microsomes, proglobeflowery acid was transformed into one product, with the molecular formula of C₁₉H₂₃O₁₀, which was supposed to be the glucuronization reaction product of proglobeflowery acid. Trollioside was transformed into two products, C₂₅H₃₆O₁₄ and C₁₈H₂₄O₉，which were supposed to be the transformation products after adding one glycosyl group and removing one methyl group, respectively. Veratric acid was transformed into one product, with the molecular formula of C₈H₈O₄, which was supposed to have two structures. It is supposed to be the product of removing one methyl group. Conclusion Glucuronidation, demethylation and glycosylation are mainly involved in the rat liver microsomal of transformation of phenolic acids from the flowers of Trollius chinensis. It provides a basis for the characterization of metabolic profiling of the phenolic components from the flowers of Trollius chinensis..

R965

北京市自然科學(xué)基金面上項(xiàng)目（7172129）