目的 探究丹參川芎嗪注射液對心腎綜合征（CRS）大鼠心腎損傷及氧化應(yīng)激反應(yīng)的影響。方法 按照隨機數(shù)字表法將大鼠分為假手術(shù)組、模型組、卡托普利（陽性藥，13.0 mg/kg）組以及丹參川芎嗪低、中、高劑量（0.4、1.0、2.0 mL/kg）組，假手術(shù)組僅暴露腎臟，不切除右腎及結(jié)扎左腎分支動脈，其余組采用永久性結(jié)扎大鼠冠狀動脈左前降支1周后行3/4腎臟切除方法建立大鼠急性CRS模型。蘇木精-伊紅（HE）染色觀察心臟、腎臟組織病理變化；化學比色法檢測各組大鼠血清血肌酐（Scr）、尿素氮（BUN）水平；ELISA法檢測血清腦鈉肽（BNP）水平以及心臟、腎臟組織中超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽過氧化物酶（GSH-px）水平。結(jié)果 與假手術(shù)組比較，模型組大鼠血清Scr、BUN、BNP水平以及心臟、腎臟組織中MDA含量均顯著升高（P<0.05），心臟、腎臟組織中SOD、GSH-px活性顯著降低（P<0.05），同時可見心肌細胞肥大、排列紊亂，部分腎小管擴張，腎小球增大、結(jié)構(gòu)模糊，刷狀緣脫落；與模型組比較，丹參川芎嗪低、中、高劑量組和卡托普利組大鼠血清Scr、BUN、BNP水平以及心臟、腎臟組織中MDA含量均顯著降低（P<0.05），心臟、腎臟組織中SOD、GSH-px活性顯著升高（P<0.05），同時心臟、腎臟組織病理損傷程度明顯改善，并呈劑量相關(guān)性。結(jié)論 丹參川芎嗪注射液可有效改善CRS誘導的心腎損傷，可能與氧化應(yīng)激反應(yīng)密切相關(guān)。

Objective To investigate the effects of Danshen Chuanxiongqin Injection on cardiorenal injury and oxidative stress in rats with cardiorenal syndrome (CRS). Methods Rats were divided into sham operation group, model group, Danshen Chuanxiongqin Injection low, medium and high dose groups and captopril group according to random number table method. The acute CRS rat model was established by 3/4 nephrectomy at one week after permanent ligation of left anterior descending coronary artery in rats. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of heart and kidney tissues. Serum Scr and BUN levels were detected by chemical colorimetry. Serum BNP level and SOD, GSH-px and MDA levels in heart and kidney tissues were detected by ELISA. Results Compared with sham-operated group, the levels of serum Scr, BUN and BNP and the content of MDA in heart and kidney tissues in model group were significantly increased (P<0.05), the activities of SOD and GSHpx in heart and kidney were significantly decreased (P<0.05). at the same time, cardiomyocyte hypertrophy, disordered arrangement, dilatation of some renal tubules, enlargement of glomerulus, blurred structure and brush-like edge shedding were observed. Compared with model group, the levels of serum Scr, BUN, BNP and the content of MDA in heart and kidney of rats in low, medium and high dose groups of Danshen Chuanxiongqin Injection and captopril groups were significantly lower (P<0.05), the activities of SOD and GSH-px in heart and kidney increased significantly (P<0.05), at the same time, the degree of pathological damage of heart and kidney was significantly improved, which were in dose-dependent manners. Conclusions Danshen Chuanxiongqin Injection can effectively improve CRS-induced heart and kidney injury, which may be closely related to oxidative stress.

R965