目的 篩選醒腦靜注射液8種單體（吉馬酮、莪術二酮、β-欖香烯、樟腦、莪術烯醇、麝香酮、天然冰片、龍腦）中抑制BV-2細胞炎癥反應的活性成分，研究其對炎癥因子釋放的影響。方法 CCK-8法檢測8種單體（10 μmol/L）對小膠質(zhì)細胞活力的影響；10 μmol/L的8種單體孵育BV-2細胞0.5 h，用0.1 μg/mL脂多糖（LPS）進行刺激，培養(yǎng)24 h后收集上清，Greiss法檢測NO濃度；Elisa檢測腫瘤壞死因子-α（TNF-α）、白介素-6（IL-6）的濃度。不同濃度的麝香酮孵育BV-2細胞0.5 h，用0.1 μg/mL LPS刺激，培養(yǎng)24 h后收集上清，Greiss法檢測NO濃度；Elisa法檢測TNF-α、IL-6、白介素1受體α（IL-1Rα）的濃度。結果 與對照組比較，醒腦靜注射液各個單體成分對正常培養(yǎng)的BV-2細胞無毒性作用。LPS刺激BV-2細胞后，模型組與對照組比較，產(chǎn)生的NO、TNF-α、IL-6、IL-1Rα顯著增多（P<0.01）；與模型組比較，麝香酮5.0、7.5、10.0 μmol/L濃度可顯著抑制NO、IL-6的產(chǎn)生，10 μmol/L麝香酮可顯著抑制TNF-α的產(chǎn)生，差異均有統(tǒng)計學意義（P<0.01）；其他7種單體成分均無顯著影響；2.5、5.0 μmol/L麝香酮組IL-1Ra的釋放量有升高趨勢，但無統(tǒng)計學意義。結論 麝香酮可以顯著抑制LPS誘導的BV-2細胞炎性因子的產(chǎn)生。

Objective To study the effect of 8 monomers of Xingnaojing injection (gematrone, zedoary diketone, β-elemene, camphor, zedoary enol, musk ketone, borneol, borneol) on the release of inflammatory factors, the active components were screened. Methods CCK-8 was used to detect the effects of eight monomers on the microglia activity. Xingnaojing eight monomers of 10 μmol/L concentration:gemone, sputum dione, β-elemene, camphor, zephyrenol, musk ketone, natural borneol, borneol to incubate BV-2 cells for 0.5 h, then stimulated with LPS. The supernatant was collected after 24 h of culture. The Greens method was used to detect the concentration of NO. Elisa detected tumor necrosis factor (TNF-α) and the concentration of interleukin-6 (IL-6). Different concentrations of musk ketone were used to incubate BV-2 cells for 0.5 h, then stimulated with lipopolysaccharide. After 24 h of culture, the supernatant was collected. CCK-8 was used to detect the effects of different concentrations of musk ketone on the microglia viability. Greiss method NO concentration was measured; Elisa detected the concentrations of tumor necrosis factor (TNF-α), interleukin 6 (IL-6), and interleukin-1 receptor alpha (IL-1Rα). Results Compared with the control group, Xingnaojing injection has no toxic effect on BV-2 cells. After LPS stimulated BV-2 cells, NO, TNF-α, IL-6 and IL-1R α were significantly increased in the model group compared with the control group (P<0.01). Compared with the model group, musk ketone of 5.0, 7.5, and 10.0 μmol/L can significantly inhibit the production of NO and IL-6, the difference was statistically significant (P<0.01). The other 7 monomers had no significant effect. The release of IL-1Ra in 10 μmol/L musk ketone group increased, but there was no statistical significance. Conclusion Musk ketone can inhibit the inflammatory activation of LPS-induced microglia and inhibit the production of inflammatory factors.

R285.5

國家自然科學基金資助項目（81573644）；國家中藥標準化項目（ZYBZH-C-JS-35）；“十三五”期間天津市高等學?！皠?chuàng)新團隊培養(yǎng)計劃”（NO.TD13-5050）