目的 研究鞣酸蛋白酵母散的治療作用及其與保護(hù)腸黏膜、促進(jìn)其修復(fù)的關(guān)系。方法 將48只21 d SD大鼠隨機(jī)分為6組：對(duì)照組、模型組、蒙脫石散（陽性藥，0.8 g/kg）組和鞣酸蛋白酵母散低、中、高劑量（0.1、0.3、0.9 g/kg）組。除對(duì)照組外，所有動(dòng)物用4 g/kg番瀉葉水煎劑連續(xù)ig 2周后，ig給藥，對(duì)照組和模型組給予等體積的蒸餾水，每天2次，給藥5 d。記錄各組大鼠稀便率和腹瀉指數(shù)。經(jīng)治療后，取各組大鼠空腸做蘇木精-伊紅染色和免疫組化，分別觀察腸黏膜組織結(jié)構(gòu)的改變和腸黏膜增殖細(xì)胞核抗原（PCNA）表達(dá)的變化。同時(shí)，刮取大鼠空腸黏膜分別測(cè)定DNA、總蛋白和IL-6的表達(dá)水平。結(jié)果 與模型組比較，經(jīng)高劑量的鞣酸蛋白酵母散治療后，大鼠稀便率和腹瀉指數(shù)顯著降低（P<0.001），空腸絨毛結(jié)構(gòu)基本恢復(fù)正常，空腸黏膜DNA含量顯著增加（P<0.05），PCNA表達(dá)顯著增加（P<0.001），IL-6表達(dá)減少。中劑量的鞣酸蛋白酵母散可改善大鼠腹瀉，但對(duì)其他指標(biāo)沒有顯著影響。而低劑量的鞣酸蛋白酵母散則無作用。結(jié)論 鞣酸蛋白酵母散對(duì)幼年大鼠腹瀉有治療作用，并可促進(jìn)腸黏膜修復(fù)。

Objective To explore the effects of Albumin Tannate and Barm Powder on diarrhea and repairing injury of intestinal mucosa. Methods Forty-eight 21-day-old rats were randomly divided into six groups:control group, diarrhea modeling group, montmorillonite powder (0.8g/kg) group, Albumin Tannate and Barm Powder low, middle, and high does (0.1, 0.3, and 0.9 g/kg) group. Except for control group, all rats were given with Senna leaf water decoction (4 g/kg) for two weeks, which was followed by treatment with corresponding drug or distilled water for 5 days. After measuring the diarrhea index, we take the jejunum for H&E stain and immunohistochemistry to evaluate tissue structure or the expression of PCNA. Besides these, scrape the intestinal mucosa to measure the level of DNA, protein and interleukin 6. Results Compared with model group, after the treatment of high dose of Albumin Tannate and Barm Powder, the defecation rate and diarrhea index of rats were significantly reduced (P<0.001), the structure of jejunum villi was basically restored to normal, the DNA content of jejunum mucosa was significantly increased (P<0.05), the expression of PCNA was significantly increased (P<0.001), and the expression of IL-6 was decreased. However, Albumin Tannate and Barm Powder of midle does could only relieve diarrhea symptom and Albumin Tannate and Barm Powder of low does had no effect on diarrhea. Conclusion Albumin Tannate and Barm Powder has good effect on diarrhea and promote the repair of intestinal mucosa.

R965