目的 運(yùn)用網(wǎng)絡(luò)藥理學(xué)探討蘇龍嘎-4顆粒的多成分、多靶點(diǎn)、多途徑的抗腹瀉作用機(jī)制。方法 通過(guò)TCMSP平臺(tái)、BATMAN-TCM數(shù)據(jù)庫(kù)及文獻(xiàn)檢索收集蘇龍嘎-4顆粒中4味中藥的全部化學(xué)成分和作用靶點(diǎn)，同時(shí)利用GeneCards數(shù)據(jù)庫(kù)和OMIM數(shù)據(jù)庫(kù)獲取腹瀉的已知治療靶點(diǎn)。構(gòu)建韋恩圖，得到蘇龍嘎-4顆?？垢篂a的作用靶點(diǎn)。采用Cytoscape 3.7.1軟件，將活性成分與潛在靶點(diǎn)構(gòu)建“藥物-成分-靶點(diǎn)”網(wǎng)絡(luò)；通過(guò)String 10.0數(shù)據(jù)庫(kù)建立PPI網(wǎng)絡(luò)，并獲得核心靶點(diǎn)。采用R軟件對(duì)交集基因進(jìn)行GO功能分析和KEGG通路分析。結(jié)果 根據(jù)數(shù)據(jù)庫(kù)檢索，結(jié)合所制定的篩選標(biāo)準(zhǔn)，得到蘇龍嘎-4顆粒34個(gè)活性成分和110個(gè)靶點(diǎn)，其中74個(gè)靶點(diǎn)為蘇龍嘎-4顆?？垢篂a的潛在靶點(diǎn)。GO生物學(xué)過(guò)程分析得出87個(gè)條目，KEGG通路分析得出117條通路，主要涉及PI3K-Akt信號(hào)通路、TNF信號(hào)通路、p53信號(hào)通路、凋亡通路等。推測(cè)蘇龍嘎-4顆粒可能通過(guò)抗炎、影響細(xì)胞凋亡、修復(fù)腸粘膜損傷而起到抗腹瀉的作用。結(jié)論 蘇龍嘎-4顆粒的活性成分主要通過(guò)CASP-3、EGFR、IL-6、VEGFA、MAPK8等靶點(diǎn)抑制炎癥因子的產(chǎn)生阻斷炎癥反應(yīng)、影響細(xì)胞凋亡的同時(shí)對(duì)腸粘膜損傷的進(jìn)行修復(fù)，最終發(fā)揮抗腹瀉的作用。

Objective Sulongga-4 Granules is a classic ethnic medicine formula, which is clinically used in the treatment of infantile diarrhea. In this study, the potential mechanism of multi-component, multi-target and multi-pathway treatment of diarrhea was explored based on network pharmacology. Methods Through TCMSP platform, Batman-TCM database and literature retrieval, all the chemical components and targets of four Traditional Chinese medicines in Sulonga-4 Granules were collected. and the known therapeutic targets of diarrhea were obtained by GeneCards database and OMIM database. The Venn diagram was constructed to obtain the therapeutic target of Sulongga-4 Granules in treating diarrhea. Cytoscape 3.7.1 software was used to construct a "drugcomponent-target" network between active ingredients and potential targets. PPI network was established through String 10.0 database and core target proteins were obtained. R software was used to analyze the GO function and KEGG pathway of intersection genes. Results According to the database search and the established screening criteria, 34 active components and 110 targets of Sulongga-4 Granules were obtained, among which 74 were potential targets for anti-diarrhea of Sulongga-4 Granules. GO biological process analysis revealed 87 items, and KEGG pathway analysis revealed 117 pathways, mainly involving PI3k-Akt signaling pathway, TNF signaling pathway, p53 signaling, pathway apoptosis pathway, etc. It is speculated that Sulongga-4 Granules may play an anti-diarrhea effect by anti-inflammatory, affecting apoptosis and repairing intestinal mucosal damage. Conclusion The active components of Sulongga-4 Granules mainly inhibit the production of inflammatory factors, block inflammatory response, promote apoptosis and repair intestinal mucosal damage through CASP-3, EGFR, IL-6, VEGFA, MAPK8 and other targets, and finally play an anti-diarrhea role.

R285.5

國(guó)家“重大新藥創(chuàng)制”重大專項(xiàng)（2017ZX09301060-013）#共同