目的 探究注射用益氣復(fù)脈（凍干）（YQFM）對阿霉素（DOX）所致大鼠急性心肌損傷保護(hù)作用組方合理性。方法 將140只雄性SD大鼠按體質(zhì)量隨機(jī)分成對照組，模型組，YQFM低、中、高劑量（0.28、0.55和1.10 g/kg）組，紅參提取物低、中、高劑量（RGE）（0.21、0.42和0.85 g/kg）組，麥冬提取物（OJE）低、中、高劑量（0.30、1.27和2.55 g/kg）組和五味子提取物（SCE）的低、中、高劑量（0.31、0.63和1.27 g/kg）組。尾iv給藥7 d，第5天一次性ip 25mg/kg DOX建立急性心肌損傷模型。觀察大鼠狀態(tài)，采集心電圖，檢測超聲心動，腹主動脈取血，取心臟稱質(zhì)量；生化儀測定血清中心肌酶水平，制作心臟切片并進(jìn)行蘇木精-伊紅（HE）染色。結(jié)果 與對照組比較，模型組大鼠精神狀態(tài)差，體質(zhì)量下降，心臟收縮功能異常，心電圖顯示T波、QT間期異常，血清中CK、LDH、AST含量均顯著上升（P<0.05），且光鏡觀察發(fā)現(xiàn)心肌細(xì)胞出現(xiàn)病理性改變。與模型組比較，各給藥組體質(zhì)量均呈明顯上升趨勢，差異顯著（P<0.05）；YQFM中、高劑量，RGE、OJE高劑量和SCE中、高劑量均顯著改善T波高聳（P<0.05），YQFM高劑量組起效程度最大；YQFM和RGE高劑量組顯著緩解QT間期延長（P<0.05）；YQFM中、高劑量組和各提取物高劑量對LVDd升高均有顯著性改善（P<0.05）；YQFM各劑量和各提取物中、高劑量均顯著提高FS（P<0.05），各給藥組均對E/A降低發(fā)揮緩解作用；YQFM各劑量均顯著降低LDH水平（P<0.05），中、高劑量組顯著降低CK、AST水平（P<0.05），各味藥提取物高劑量顯著降低CK、LDH和AST水平（P<0.05），SCE中劑量顯著降低LDH和AST水平（P<0.05）；YQFM中、高劑量明顯改善心肌病理性變化。結(jié)論 紅參、麥冬、五味子均為保護(hù)急性心肌損傷的藥效組分，組合用藥能夠降低起效濃度，YQFM組方具有一定的科學(xué)性。

Objective Rationality of Yiqi Fumai Lyophilized Injection against doxorubicin induced acute myocardial injury in rats. Methods 140 male SD rats were randomly divided into control group, model group, YQFM low, medium and high dose (0.28, 0.55 and 1.10 g/kg), red ginseng extract (RGE) (0.21, 0.42 and 0.85 g/kg) groups, and Ophiopogon japonicus extract (OJE) low, medium and high dose (0.30, 1.27 and 2.55 g/kg), and Schisandra chinensis extract (SCE) low, medium and high dose (0.31, 0.63 and 1.27 g/kg) groups. Treatment of the rats with relative drugs for seven days continuously and inducing acute myocardial injury by injecting DOX on the fifth day. The status of the rats was observed, electrocardiogram andechocardiography were performed, blood and heart were collected. The relevant enzyme content in the serum was detected and heart sections were stained with hematoxylin-eosin (HE). Results Compared with control group, the systolic dysfunction was abnormal in model group, the mental state of the rats was poor, the electrocardiogram T wave and QT interval were abnormal, and the serum levels of CK, LDH and AST were all increased significantly (P<0.05), and light pathological observation showed pathological changes in the control group. Compared with the model group, the body weight of each treatment group showed an upward trend, the difference was significant (P<0.05); YQFM medium and high dose, RGE, OJE high dose and SCE medium and high dose all significantly improved T wave towering (P<0.05), YQFM high dose group significantly alleviated QT interval prolongation (P<0.05); YQFM middle and high dose group and each extract high dose group had significant effect on LVDd increased (P<0.05). All YQFM doses, middle and high dose extracts of YQFM significantly increased FS (P<0.05), and each dose of YQFM significantly reduced the level of LDH (P<0.05), the levels of CK and AST in medium and high dose groups were significantly decreased (P<0.05), and the high dose of extract significantly reduced CK, LDH and AST. The levels of LDH and AST were significantly decreased by SCE medium dose (P<0.05), and the myocardial pathological changes were significantly improved by medium and high dose YQFM. Conclusion RGE, OJE and SCE are all effective components and the combination of drugs can take effects in a lower concentration works. Therefore, YQFM against myocardial injury is rational.

R965

天津市科技計劃項目（18YFCZZC00430、17PTSYJC00090）