目的 應(yīng)用Micro-CT技術(shù)觀察淫羊藿苷和朝藿定C對糖皮質(zhì)激素性骨質(zhì)疏松癥（GIOP）小鼠模型骨組織骨量、骨微結(jié)構(gòu)的影響。方法 8周齡C57/BL6雄性小鼠隨機(jī)分為4組：對照組、模型組、淫羊藿苷（200 mg/kg）組和朝藿定C（200 mg/kg）組，除對照組外，其他3組小鼠im地塞米松5 mg/kg，0.125 mg/次，每周3次，制備GIOP模型，對照組im等體積的生理鹽水，造模同時，每天ig給藥1次，連續(xù)60 d后取材，采用micro-CT方法對脛骨近端骨微結(jié)構(gòu)進(jìn)行三維分析；HE染色病理切片觀察脛骨近端骨組織病理形態(tài)。結(jié)果 骨量參數(shù)：與對照組比較，模型組骨礦物質(zhì)含量（BMC）、骨礦物質(zhì)密度（BMD）、組織礦物含量（TMC）、組織礦物質(zhì)密度（TMD）均顯著下降（P<0.05、0.01）；與模型組比較，淫羊藿苷組、朝藿定C組BMC、BMD、TMC、TMD指標(biāo)均顯著提高（P<0.05）；其中朝藿定C組各指標(biāo)均較淫羊藿苷組顯著升高（P<0.05）。與對照組比較，模型組相對骨體積（BV/TV）、骨小梁數(shù)量（Tb.N）、骨小梁厚度（Tb.Th）指標(biāo)均顯著下降（P<0.05），骨小梁分離度（Tb.Sp）、結(jié)構(gòu)模型指數(shù)（SMI）均顯著提高（P<0.05）；與模型組比較，淫羊藿苷、朝藿定C組BV/TV、Tb.N、Tb.Th顯著升高，Tb.Sp、SMI顯著下降（P<0.05），其中朝藿定C組各指標(biāo)均較淫羊藿苷組改善顯著（P<0.05）。HE染色病理切片示，模型組骨小梁數(shù)目明顯減少，稀疏斷裂，大部分不能連接成網(wǎng)狀，骨髓腔明顯增大，骨小梁結(jié)構(gòu)出現(xiàn)較大的空白區(qū)域；淫羊藿苷及朝藿定C組小鼠骨小梁明顯寬厚，數(shù)目也顯著增加，骨小梁斷裂較少，骨小梁光滑，接近對照組，其中朝藿定C組增加較淫羊藿苷組明顯。結(jié)論 地塞米松誘導(dǎo)的骨質(zhì)疏松小鼠模型成功建立，朝藿定C和淫羊藿苷抗骨質(zhì)疏松活性明顯，主要通過增加骨量和改善骨小梁微結(jié)構(gòu)來最終提高骨強(qiáng)度，其中朝藿定C抗骨松作用更強(qiáng)；Micro-CT技術(shù)與傳統(tǒng)的檢測方法相比，在中藥干預(yù)GIOP骨微結(jié)構(gòu)參數(shù)分析上具有便捷、高效，經(jīng)濟(jì)，圖像多維、全面，準(zhǔn)確的優(yōu)勢。

Objective Micro-CT technique was used to observe the effects of icariin and epimedin C on bone mass and bone microstructure in the mouse model of glucocorticoid osteoporosis (GIOP). Methods Tatolly 40 C57/BL6 male mice of 8-week-old were randomly divided into four groups:control group, model group, icariin (200 mg/kg) group, and epimedin C (200 mg/kg) group. Except the control group, the other three groups were injected with dexamethasone 5 mg/kg, 0.125mg/time, three times a week, and the control group was injected with physiological saline of equal volume. At the same time, the drugs were gave by gavage once a day, and the samples were taken after 60 days. The microstructure of the proximal tibia was analyzed using Micro-CT method. The histopathology of the proximal tibia was observed by HE staining. Results Bone mass parameters:Compared with the control group, the BMC, BMD, TMC, and TMD indexes of the model group decreased significantly (P<0.01). Compared with the model group, the BMC, BMD, TMC and TMD indexes of the icariin group and the epimedin C group increased significantly. The index of epimedin group C was higher than that of icariin group. Bone microstructure parameters:compared with the control group, the indexes of BV/TV, TB.N, TB.SP and SMI in the model group decreased significantly, TB.SP and SMI increased significantly (P<0.05). Compared with the model group, the indexes of BV/TV, TB.N and TB.Th in the icariin group and epimedin C group increased, and the indexes of TB.SP and SMI decreased significantly (P<0.05). After HE staining pathological section in the model group, the number of bone trabeculae was significantly reduced, with sparse and broken pattern; most of them could not be connected into a network; the bone marrow cavity was significantly increased; the bone trabeculae structure appeared a large blank area. In the icariin and epimedin C group, similar to the control group, the bone trabeculae were obviously wider and thicker; the number was also noticeably increased; the bone trabeculae were less broken; the bone trabeculae were smooth. Moreover, epimedin C group increased more obviously than the icariin group. Conclusion Dexamethasone induced osteoporosis mouse model was established successfully. The anti-osteoporosis activity of epimedin C and icariin was obvious, and bone mass and bone trabecular microstructure were significantly improved, leading to the improvement of bone strength. Among them, epimedin C had stronger anti-osteoporosis effect. Compared with the traditional detection method, Micro-CT technology was convenient and efficient in the evaluation of GIOP bone microstructure parameters of traditional Chinese medicines.

R285.5

湖北省自然科學(xué)基金資助項目（2018CFB695）；廣東省應(yīng)用植物學(xué)重點實驗室開放課題（AB2018027）；中國科學(xué)院華南農(nóng)業(yè)植物分了分析與遺傳改良重點實驗室開放課題（KF202002）