目的 基于網(wǎng)絡(luò)藥理學(xué)的方法，分析加味丹參飲對于治療心肌梗死的潛在作用機制。方法 借助TCMSP、Drugbank、Pubchem、Swiss Target Prediction等數(shù)據(jù)庫及參考文獻獲取加味丹參飲的有效成分及靶標。通過HPO、OMIM平臺檢索心肌梗死疾病相關(guān)的靶標，并借助STRING數(shù)據(jù)庫構(gòu)建靶蛋白相互作用網(wǎng)絡(luò)（PPI），獲得關(guān)鍵靶標。使用Cytoscape3.6.0軟件對加味丹參飲-心肌梗死疾病進行網(wǎng)絡(luò)可視化，并構(gòu)建“加味丹參飲-化學(xué)成分-靶點”、“疾病-靶點”、“加味丹參飲-相關(guān)化學(xué)成分-疾病-關(guān)鍵靶點”網(wǎng)絡(luò)圖，使用DAVID數(shù)據(jù)庫對關(guān)鍵靶點進行GO生物過程和KEGG代謝通路分析。結(jié)果 從加味丹參飲中共篩選出94個有效成分，148個與心肌梗死相關(guān)的作用靶點，其中有效成分與疾病對應(yīng)的相同靶點21個，其中包含血管內(nèi)皮生長因子A（VEGFA）、酪氨酸激酶2（JAK2）、腫瘤壞死因子（TNF）等。經(jīng)GO和KEGG通路富集分析，靶點主要涉及G蛋白偶聯(lián)受體信號通路、調(diào)節(jié)細胞增殖、RNA聚合酶II轉(zhuǎn)錄因子活性等功能過程，可能通過調(diào)節(jié)PI3K-Akt信號通路、微小RNA-癌癥相關(guān)通路、AMPK信號通路、VEGF信號通路、JAK-STAT信號通路等多條代謝通路來發(fā)揮加味丹參飲治療心肌梗死的作用。結(jié)論 通過建立靶點網(wǎng)絡(luò)圖，可從多方位闡明復(fù)方加味丹參飲治療心肌梗死多成分、多靶點的機制，可進一步為深入研究加味丹參飲治療心肌梗死相關(guān)疾病機制提供理論依據(jù)。

Objective Based on the method of network pharmacology, the potential mechanism of Jiawei Danshen Decoction in the treatment of myocardial infarction was analyzed. Methods Tcmsp, drugbank, PubChem, Swiss target prediction and other databases and references were used to obtain the effective components and targets of Jiawei Danshen Decoction. The targets related to myocardial infarction were searched by HPO and OMIM platforms, and the PPI was constructed with STRING database to obtain the key targets. Using the software of Cytoscape 3.6.0, the network visualization of Jiawei Danshen Decoction-myocardial infarction disease was carried out, and the network diagram of "Jiawei Danshen Decoction-chemical composition target", "disease-target" and "Jiawei Danshen Decoction-related chemical components-disease-key target" was constructed. DAVID database was used to analyze the GO biological process and KEGG metabolic pathway of key targets. Results A total of 94 active components, and 148 targets related to myocardial infarction were screened out from Jiawei Danshen Decoction. Among them, there were 21 targets corresponding to the disease, VEGFA, JAK2, TNF, etc. After enrichment analysis of GO and KEGG pathways, the target proteins mainly involved in G protein coupled receptor signaling pathway, cell proliferation regulation, RNA polymerase II transcription factor activity and other functional processes, it may play a role in treating myocardial infarction by regulating PI3K Akt signaling pathway, microRNA cancer related pathway, AMPK signaling pathway, VEGF signaling pathway, and JAK-STAT signaling pathway. Conclusion Through the establishment of target network map, we can clarify the mechanism of multicomponent and multi-target of Jiawei Danshen Decoction in treatment of myocardial infarction, and provide theoretical basis for further research on the mechanism of Jiawei Danshen Decoction in treating myocardial infarction related diseases.

R285.5

國家自然科學(xué)基金青年項目（81704065）；湖南省中醫(yī)藥科研項目計劃重點項目（202016）；湖南省自然科學(xué)基金面上項目（2020JJ4466）；中醫(yī)內(nèi)科學(xué)省部級共建教育部重點實驗室開放基金（ZYNK201401）；湖南省教育廳科學(xué)研究優(yōu)秀青年項目（16B198）；湖南省自然科學(xué)基金青年項目（2017JJ3239）；湖南省中醫(yī)藥科研計劃項目（201790）；中醫(yī)內(nèi)科學(xué)省部共建教育部重點實驗室開放基金重點項目（ZYFT201507）；湖南省自然科學(xué)基金-青年基金項目（2019JJ50471）。