目的 基于網(wǎng)絡(luò)藥理學(xué)探討棗仁安神顆粒治療失眠的作用機(jī)制。方法 通過(guò)查閱相關(guān)文獻(xiàn)，挖掘棗仁安神顆粒的入血成分，并采用反向分子對(duì)接，預(yù)測(cè)入血成分的相關(guān)靶點(diǎn)，與失眠靶點(diǎn)進(jìn)行交集映射，獲得其治療失眠的作用靶點(diǎn)。利用蛋白互作篩選核心靶點(diǎn)，并進(jìn)行分子對(duì)接驗(yàn)證?；诠灿邪悬c(diǎn)，采用功能注釋生物信息學(xué)芯片進(jìn)行分析，得到棗仁安神顆粒治療失眠的關(guān)鍵通路。結(jié)果 方中3味中藥酸棗仁、丹參、北五味子共得到29個(gè)入血成分和184個(gè)潛在靶點(diǎn)，其中19個(gè)成分、60個(gè)靶點(diǎn)和36條通路與失眠極為密切，主要涉及神經(jīng)活性配體-受體相互作用、色氨酸代謝、5-羥色胺能突觸、GABA能突觸等相關(guān)分子環(huán)節(jié)。結(jié)論 方中3味中藥以作用靶點(diǎn)為媒介，連接入血成分及相關(guān)通路，從而發(fā)揮抗失眠的生物學(xué)效應(yīng)，體現(xiàn)了中藥多成分、多靶點(diǎn)，多途徑協(xié)同起效的作用特點(diǎn)。

Objective The mechanism of the treatment of insomnia by Zaoren Anshen Granules was discussed based on the network pharmacology. Methods By consulting the relevant literatures, the blood components of Zaoren anshen granules were excavated. Reverse molecular docking was used to predict the relevant targets of chemical components, Potential targets of Zaoren anshen granules were obtained by mapping with targets of insomnia. Subsequently, protein-protein interactions were used to screen core targets and molecular docking validation was performed. Based on the above-mentioned common targets, functional annotation bioinformatics chips were used to analyze the key pathways for the treatment of insomnia by Zaoren anshen granules, and to explore the possible mechanism of treatmenting insomnia. Results Three traditional Chinese medicines, 29 blood components and 184 potential targets were obtained, of which 19 components and 60 targets and 36 pathways were related to insomnia, mainly related to neuroactive ligand-receptor interaction, tryptophan metabolism, serotonin synapses, GABA ergic synapses and other related molecular links. Conclusion The targets were used as a medium to connect blood components and related pathways, thus exerting the biological effect of insomnia, reflecting the multi-components, multi-targets and multi-pathways synergistic action of traditional Chinese medicine.

R285.5

國(guó)家自然科學(xué)基金資助項(xiàng)目（81173541）