目的 探究開竅藥蘇合香對腦缺血再灌注大鼠的腦損傷及血腦屏障通透性的影響。方法 健康成年雄性Wistar大鼠隨機分為3組：假手術(shù)組、模型組、蘇合香（0.4 g/kg）組。線栓法建立短暫性大腦中動脈閉塞模型，于腦缺血2 h后ig給藥，檢測大鼠腦缺血后神經(jīng)功能評分，計算大鼠腦含水量及腦梗死率，檢測大鼠血常規(guī)及鼠尾出血時間。應(yīng)用伊文思藍染色法檢測大鼠生理狀態(tài)以及腦缺血后6、12、24、48、72 h血腦屏障通透性的變化。采用凝血四項試劑盒檢測大鼠腦缺血6、12、24、48、72 h的凝血功能。結(jié)果 與模型組比較，蘇合香顯著改善腦缺血大鼠神經(jīng)行為學(xué)評分（P<0.05），顯著降低腦梗死率（P<0.05），顯著延長鼠尾出血時間（P<0.05），但對腦含水量及血常規(guī)無明顯影響；血腦屏障通透性實驗結(jié)果顯示，蘇合香能夠開放正常大鼠血腦屏障（P<0.01）；與模型組比較，蘇合香能增強腦缺血再灌注大鼠血腦屏障通透性，且缺血48 h時最為顯著（P<0.01）；另外蘇合香可顯著延長腦缺血24 h時大鼠凝血酶原時間（PT）、凝血活酶時間（TT），降低纖維蛋白原（FIB）含量（P<0.05）。結(jié)論 蘇合香對腦缺血再灌注損傷大鼠具有明顯腦保護作用，同時可能通過影響凝血功能增加正常及缺血狀態(tài)下血腦屏障通透性，為蘇合香腦保護作用研究提供了新的線索。

Objective To explore the effects of storax on brain protection and blood-brain barrier (BBB) permeability of rats with cerebral ischemia-reperfusion (I/R) injury. Methods Wistar male rats were randomly divided into three groups:Sham group, model group, and storax (0.4 g/kg) group. The model of transient middle cerebral artery occlusion (tMCAO) was established by sutureoccluded method, rats were ig with drugs two hours after cerebral ischemia, then neurological function scores, cerebral infarction rate and cerebral edema of rats after cerebral ischemia were measured, and the blood routine and the tail bleeding time of the rats were measured as well. Evans blue staining method was used to detect the physiological status of rats and the changes in BBB permeability of 6, 12, 24, 48, and 72 h cerebral ischemia. Coagulation four test kit were used to detect the coagulation function of rats with cerebral ischemia at 6, 12, 24, 48, 72 h. Results Compared with model group, storax can significantly improve the neurobehavioral scores (P<0.05), reduce the rate of cerebral infarction (P<0.05), and prolong the bleeding time of rats tail (P<0.05), but it had no obvious effect on the brain water content and blood test; Storax can increase the BBB permeability of physiological rats with the increasing time and compared with the model group, the permeability of BBB could be enhanced by storax, which was most significant at 48 h (P<0.01). In addition, storax can prolong the prothrombin time (PT) and prothrombin time (TT), and decreased the fibrinogen (FIB) content after ischemic stroke of rats at 24 h (P<0.05). Conclusion Storax has obvious neuroprotective effect on rats with cerebral I/R injury, and increase the permeability of the BBB under normal and ischemic conditions, which may be related to prolonged blood coagulation and anti-platelet aggregation.

R285.2

國家自然科學(xué)基金青年項目（81704019）