目的 建立瀉白散和方中3味主藥甘草、地骨皮、桑白皮的體外抗氧化活性測定方法，并對31批藥材和10批瀉白散煎液的抗氧化活性進(jìn)行測定。方法 采用紫外可見分光光度法檢測一定濃度的藥材提取液引起DPPH溶液吸光度（A）值降低，考察波長為517 nm，分別探索3味藥材抗氧化活性成分的提取條件；并進(jìn)行不同溶劑的吸收考察、專屬性考察、DPPH線性考察、藥材提取液線性考察、精密度試驗(yàn)、重復(fù)性試驗(yàn)、耐用性考察等方法學(xué)驗(yàn)證；以清除DPPH自由基的半抑制濃度（IC₅₀）作為評價(jià)指標(biāo)，對瀉白散和方中3味藥材的體外抗氧化活性進(jìn)行考察。結(jié)果 地骨皮、甘草、桑白皮和瀉白散提取液的IC₅₀均值為0.31、1.24、1.49和0.91 g/L，瀉白散提取工藝對方中藥物抗氧化活性的保留均值為56%。結(jié)論 建立的抗氧化活性測定方法可用于瀉白散及方中主藥的抗氧化活性測定，為多維度評價(jià)中藥和中藥材質(zhì)量提供新思路。

Objective To establish a method for the determination of antioxidant activity of Xiebai San and three main compositions of it in vitro, determine the antioxidant activity of 31 batch samples of CMM and 10 batch samples of Xiebai San. Methods To explore the extraction conditions of antioxidative components from 3 CMM, the decrease of DPPH solution absorption value caused by a certain concentration CMM solution was taken as the evaluation index (change of absorption value/concentration of CMM); the blank absorption, specificity, DPPH linearity, herbal extract linearity, precision test, repeatability test and durability test were used to verify the methodology. The IC₅₀ of cleared DPPH free radicals was used as the index to evaluate the antioxidant activity in vitro of Xiebai San and three CMM. Results The mean IC₅₀ of Lycii Cortex, Glycyrrhizae Radix et Rhizoma, Mori Cortex and Xiebai San were 0.31, 1.24, 1.49 and 0.91 g/L; The mean value of the antioxidant activity of the extract from Xiebai San was 56%. Conclusion The method was applicable to determine the antioxidant activity of Xiebai San and the main compositions of it in vitro. And it provides new idea for multi-dimensional evaluation the quality of traditional Chinese medicine and CMM.

R962.2

天津市科技重大專項(xiàng)與工程（18ZXXYSY00020）