目的 研究牙痛停滴丸對大鼠口腔潰瘍的治療效果，并設(shè)計小鼠耳腫脹實驗驗證其抗炎作用。方法 SD大鼠隨機(jī)分為對照組、模型組、牙痛停滴丸組（86.4 mg/kg，4倍臨床等效劑量）、甲硝唑組（16.2 mg/kg，臨床等效劑量，陽性藥），每組10只。除對照組外，以80%冰醋酸溶液灼燒頰側(cè)口腔黏膜的方法建立口腔潰瘍模型，造模24 h開始給藥，連續(xù)ig給藥10 d，模型組和對照組給予等量蒸餾水。觀察潰瘍大體形態(tài)；于造模1 d和給藥4、10 d，利用游標(biāo)卡尺測量大鼠口腔潰瘍的直徑大小，進(jìn)行口腔潰瘍評分；于給藥第4天，取每組2只大鼠潰瘍處口腔黏膜做病理組織學(xué)觀察。ICR小鼠隨機(jī)分為模型組、阿司匹林（195 mg/kg，臨床等效劑量，陽性藥）組、牙痛停滴丸（124.8 mg/kg，4倍臨床等效劑量）組，每天給藥1次，連續(xù)ig 3 d。于末次給藥1 h后，以二甲苯致小鼠耳腫脹為炎癥模型，考察小鼠耳片的腫脹度。結(jié)果 大體觀察顯示，給藥10 d，模型組潰瘍表面仍存在假膜，牙痛停滴丸組基本愈合，甲硝唑組潰瘍面積減小并伴有假膜；與模型組比較，給藥4、10 d時，牙痛停滴丸和甲硝唑組潰瘍直徑顯著減小（P<0.05），口腔潰瘍評分顯著降低（P<0.05）；HE染色顯示，牙痛停滴丸組假膜較模型組小，且鱗狀上皮細(xì)胞壞死程度較低。與模型組比較，牙痛停滴丸及阿司匹林組的小鼠耳片腫脹度均顯著降低（P<0.05、0.01）。結(jié)論 牙痛停滴丸可以治療炎癥、促進(jìn)口腔潰瘍的愈合，緩解潰瘍表面的紅腫情況，對口腔潰瘍有一定的治療作用。

Objective To study the therapeutic effect of Yatongting Dropping Pills (YTTDP) on oral ulcer in rats, and design mouse ear swelling experiment to verify its anti-inflammatory effect. Methods SD rats were randomly divided into control group, model group, YTTDP group (86.4 mg/kg, four times of clinical equivalent dose) and metronidazole group (16.2 mg/kg, clinical equivalent dose, positive drug), with 10 rats in each group. Expect for the control group, the buccal oral ulcer model was established by burning buccal mucosa with 80% glacial acetic acid solution. After 24 h of modeling, the drug was ig given continuously for 10 days. The model group and the control group were given the same amount of distilled water. The general morphology of ulcer was observed; the diameter of oral ulcer was measured by vernier caliper on the 1st day of modeling and the 4th and 10th days of administration, and the score of oral ulcer was made; on the 4th day of administration, the oral mucosa of two rats in each group was taken for histopathological observation. ICR mice were randomly divided into model group, aspirin (195 mg/kg, clinical equivalent dose, positive drug) group and YTTDP (124.8 mg/kg, four times clinical equivalent dose) group, the drug was ig given continuously for three dags. One hour after the last administration, xylene induced ear swelling in mice was used as the inflammatory model to investigate the swelling degree of mouse ear piece. Results Gross observation showed that there was still pseudomembrane on the ulcer surface in the model group after 10 days of administration, which was basically healed in the YTTDP group, and the ulcer area was reduced and accompanied with pseudomembrane in the metronidazole group. Compared with model group, the ulcer diameter and oral ulcer score of YTTDP and metronidazole groups were significantly decreased (P<0.05). HE staining showed that the pseudomembrane of YTTDP group was smaller than that of model group, and the necrosis degree of squamous epithelial cells was lower. Compared with model group, the ear swelling degree of mice in YTTDP and aspirin groups were significantly decreased (P < 0.05, 0.01). Conclusions YTTDP can treat inflammation, promote the healing of oral ulcers, relieve the redness on the surface of ulcers, and have a certain therapeutic effect on oral ulcers.

R285.5

“十三五”天津市高等學(xué)?！皠?chuàng)新團(tuán)隊培養(yǎng)計劃”基金資助項目（TD13-5050）