目的 建立白及Bletilla striata（Thunb.） Reichb.f.藥材UPLC指紋圖譜，分析云南、貴州產(chǎn)白及藥材大小與化學(xué)成分間的關(guān)聯(lián)關(guān)系，為兩地白及藥材的質(zhì)量評價提供依據(jù)。方法 依據(jù)白及單個塊莖的質(zhì)量將云南、貴州產(chǎn)白及劃分為大（質(zhì)量≥ 10 g）、中（5 g ≤質(zhì)量<10 g）、?。ㄙ|(zhì)量<5 g）3類，建立以兒茶素為內(nèi)標、可進行半定量分析的白及UPLC指紋圖譜測定方法，測定兩產(chǎn)地不同大小的白及指紋圖譜。通過對指紋圖譜的相似度分析、聚類分析、熱圖分析，Spearman相關(guān)性分析，探討兩產(chǎn)地白及藥材大小與化學(xué)成分間的關(guān)系。結(jié)果 云南、貴州兩地的UPLC指紋圖譜共有14個共有峰；17批云南產(chǎn)白及的相似度均在0.85以上，22批貴州產(chǎn)白及的相似度均在0.8以上，而兩產(chǎn)地對照指紋圖譜間的相似度較低（0.760），且云南產(chǎn)白及譜圖缺少16、17號峰；將兩產(chǎn)地相同規(guī)格的樣品進行比較，貴州產(chǎn)白及的共有峰成分含量大于云南產(chǎn)白及；兩產(chǎn)地指紋圖譜中1、2、5、8、9、10、12、13、15號共有峰化學(xué)成分含量與白及大小呈負相關(guān)（P<0.05，0.4<r ≤ 0.8），其中8、9、10號峰與白及大小的相關(guān)性較強（r>0.7）。結(jié)論 云南和貴州產(chǎn)地白及藥材中化學(xué)成分及其含量存在明顯差異，且同一產(chǎn)地白及大小與1、2、5、8、9、10、12、13、15號峰化學(xué)成分呈負相關(guān)。除外在性狀外，建議考慮將產(chǎn)地作為白及藥材規(guī)格等級劃分的指標之一，為兩地白及藥材質(zhì)量優(yōu)劣的評價提供參考。

Objective To establish the UPLC fingerprints for analyzing the relationship between the size and chemical constituents content of Bletillae Rhizoma from Yunnan and Guizhou, and provide a basis for quality control of Bletillae Rhizoma. Methods Bletillae Rhizoma collected from Yunnan and Guizhou were divided into three categories (large:m ≥ 10 g, medium:5 g ≤ m < 10 g and small:m < 5 g) based on the tuber weight. The UPLC fingerprints were developed by using catechin as an internal standard, which can be used for semi-quantitative analysis. Similarity analysis, cluster analysis, heat map analysis, and Spearman correlation analysis were used to analyze the relationship between the size and chemical constituents content of Bletillae Rhizoma from the two origins. Results The 14 common peaks were marked in the fingerprint of Bletillae Rhizoma. The similarity of 17 batches of Bletillae Rhizoma from Yunnan was above 0.85, and the similarity of 22 batches of Bletillae Rhizoma from Guizhou was above 0.8. The similarity of the control fingerprints between the two regions was lower as 0.760, and the fingerprints of Bletillae Rhizoma produced in Yunnan lacked peaks 16 and 17. The content of the same components in samples from Guizhou were usually higher than that from Yunnan with the same size. The content of the most common peaks (No. 1, 2, 5, 8, 9, 10, 12, 13, and 15) in the fingerprints is negatively correlated with the size of Bletillae Rhizoma (P<0.05, 0.4 < r ≤ 0.8), and peaks 8, 9, and 10 have a strong correlation with the size of Bletillae Rhizoma (r>0.7). Conclusion There are obvious differences in the chemical constituents of Bletillae Rhizoma from Yunnan and Guizhou. And the size of Bletillae Rhizoma in the same origin has a negative correlation with the chemical constituents content. Therefore, in addition to the size of Bletillae Rhizoma, the origin of Bletillae Rhizoma should also be considered as one of the factors for the quality classification of Bletillae Rhizoma, which provides a reference for the evaluation of the quality of Bletillae Rhizoma from Yunnan and Guizhou.

R917

國家發(fā)改委中醫(yī)藥管理局中藥標準化項目（ZYBZH-Y-HUB-20）