目的 通過網(wǎng)絡(luò)藥理學(xué)的方法，篩選黃芩Scutellariae Radix的主要活性成分，構(gòu)建藥物-成分-疾病-靶點(diǎn)網(wǎng)絡(luò)，探討其治療鼻咽癌分子機(jī)制。方法 采用TCMSP、String及Genecard等數(shù)據(jù)庫，獲取黃芩化學(xué)成分及其相關(guān)靶點(diǎn)、鼻咽癌疾病的靶點(diǎn)，并制作蛋白互作網(wǎng)絡(luò)。通過Cytoscape 3.7.2繪制藥物-成分-疾病-靶點(diǎn)網(wǎng)絡(luò)?；谏镄畔W(xué)開源軟件Bioconductor以及R統(tǒng)計(jì)程序設(shè)計(jì)語言對黃芩作用的生物學(xué)過程以及KEGG進(jìn)行富集分析。結(jié)果 黃芩的36個(gè)有效成分通過調(diào)控48個(gè)靶點(diǎn)和99條通路對鼻咽癌進(jìn)行治療，4個(gè)關(guān)鍵的化合物為漢黃芩素、黃芩素、刺槐素及β-谷甾醇，可通過AKT1、JUN、CASP3、VEGFA等關(guān)鍵靶蛋白介導(dǎo)AGE-RAGE、乙型肝炎、白細(xì)胞介素17等信號通路發(fā)揮抗鼻咽癌作用。結(jié)論 黃芩可以通過多靶點(diǎn)、多途徑參與鼻咽癌的治療。

Objective To investigate the potential therapy targets and pharmacological mechanism of Scutellariae Radix in nasopharyngeal carcinoma based on network pharmacology. Methods Drug ingredients-target network was constructed based on the target sets of Scutellariae Radix and nasopharyngeal carcinoma. The overlapping genes between Scutellariae Radix targets and nasopharyngeal carcinoma-related molecular targets were investigated using Cytoscape3.7.2. Furthermore, the PPI network and module was constructed based on overlapping genes and hub nodes, respectively, followed by the pathway enrichment analysis. Results The 36 active components of Scutellariae Radix can regulate 48 targets and 99 pathways to treat nasopharyngeal carcinoma. Four key compounds are wogonin, baicalein, robinia pseudoacacia and β sitosterol. They can mediate AGE-RAGE, hepatitis B and interleukin-17 signaling pathways through AKT1, Jun, CASP3 and VEGFA. Conclusion Scutellariae Radix can participate in the treatment of nasopharyngeal carcinoma through multiple targets and pathways.

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