目的完善ICR小鼠背景數(shù)據(jù)。方法取48只正常青年ICR小鼠，動(dòng)物飼養(yǎng)1周后實(shí)施安樂(lè)死術(shù)并進(jìn)行大體解剖觀察，收集心臟、肝臟、脾臟、肺、腎臟等40多個(gè)臟器固定于10%中性緩沖福爾馬林液中，完成石蠟切片制作后進(jìn)行HE染色及組織病理學(xué)觀察。結(jié)果所有小鼠大體解剖未見(jiàn)任何異常。所有鏡下組織病理學(xué)改變均考慮為該系小鼠自發(fā)性病理改變。僅1例小鼠肺臟可見(jiàn)灶性增生性改變。低倍鏡下可見(jiàn)肺實(shí)質(zhì)中存在似島嶼樣、細(xì)胞排列緊密的實(shí)體型嗜酸性組織團(tuán)塊，與周圍組織界限清晰，組織團(tuán)塊對(duì)周圍肺泡組織存在輕微的擠壓，周圍組織未見(jiàn)病理改變。高倍鏡下可見(jiàn)團(tuán)塊中的細(xì)胞分化良好，胞核藍(lán)染呈圓形、卵圓形、梭形或不規(guī)則形，核仁不明顯，核分裂象少見(jiàn)；胞質(zhì)豐富呈嗜酸性粉染，可見(jiàn)微小空泡；細(xì)胞之間界限模糊，細(xì)胞呈腺樣排列。將該例小鼠的肺部病灶診斷為支氣管-肺泡型肺腺瘤。結(jié)論這是首次在5~6周齡青年ICR小鼠中對(duì)肺腺瘤的報(bào)道，該例自發(fā)性肺腺瘤將ICR小鼠肺腫瘤的最早發(fā)生點(diǎn)定位到了5~6周齡。

Objectives To enrich the background changes of ICR mice. Methods Forty eight healthy young ICR mice were euthanized and observed for gross anatomy after one week. More than 40 organs including heart, liver, spleen, lung and kidney were collected and fixed in 10% neutral buffered formalin solution. Paraffin sections were made for HE staining and histopathological observation. Results The gross anatomy of all mice was normal. All the histopathological changes were considered as spontaneous pathological changes. Only one mouse lung showed focal proliferative changes. Under the low power microscope, there were island like solid eosinophilic tissue masses with closely arranged cells in the lung parenchyma, with clear boundaries with the surrounding tissues. The tissue masses had slight compression on the surrounding alveolar tissues, and no pathological changes were found in the surrounding tissues. At high magnification, the cells in the mass were well differentiated with round, oval, fusiform or irregular nuclei stained with blue, unclear nucleoli and rare mitotic figures; the cytoplasm was abundant with eosinophilic powder staining and tiny vacuoles; the boundaries between cells were blurred and the cells were arranged in adenoid. The lung lesion of this mouse was diagnosed as bronchoalveolar pulmonary adenoma. Conclusion To the best of our knowledge, this is the first case report of the lung A/B adenoma in the young ICR mice and we reset the time of commence of the lung adenoma in ICR mice as young as 5～6 weeks old.

Q95-3

國(guó)家科技重大專項(xiàng)重大新藥創(chuàng)制（2018ZX09721004-001-003）