目的探討大蒜素聯(lián)合順鉑對人宮頸癌HeLa細(xì)胞增殖的抑制作用并探討其機制。方法取對數(shù)生長期HeLa細(xì)胞，設(shè)對照組、大蒜素（50 μg/mL）組、順鉑（5 μg/mL）組、聯(lián)合給藥組（大蒜素50 μg/mL+順鉑5 μg/mL）。藥物干預(yù)48 h后，通過MTT法檢測細(xì)胞增殖抑制率，運用CompuSyn軟件計算大蒜素與順鉑聯(lián)合指數(shù)（CI），流式細(xì)胞術(shù)檢測細(xì)胞周期分布和細(xì)胞凋亡水平，Western blotting法檢測Cyclin D1、CDK2、P27、Cleaved Caspase-3、Bcl-2、Bax蛋白表達(dá)。結(jié)果與大蒜素組和順鉑組比較，聯(lián)合給藥組HeLa細(xì)胞增殖抑制率顯著升高（P<0.05、0.01），CI為0.69（提示大蒜素與順鉑具有中度協(xié)同效應(yīng)）。與對照組比較，大蒜素組和順鉑組G₀/G₁期細(xì)胞比例和細(xì)胞凋亡率顯著升高（P<0.05、0.01），Cyclin D1、CDK2、Bcl-2表達(dá)顯著下調(diào)且P27、Cleaved Caspase-3、Bax表達(dá)顯著上調(diào)（P<0.05、0.01），Bax/Bcl-2比值顯著升高（P<0.01）。與大蒜素組和順鉑組比較，聯(lián)合給藥組G₀/G₁期細(xì)胞比例和細(xì)胞凋亡率顯著升高（P<0.05、0.01），Cyclin D1表達(dá)顯著下調(diào)且P27、Cleaved Caspase-3、Bax表達(dá)顯著上調(diào)（P<0.05、0.01），Bax/Bcl-2值顯著升高（P<0.01）。結(jié)論大蒜素聯(lián)合順鉑具有協(xié)同抑制人宮頸癌HeLa細(xì)胞增殖的作用，可能與調(diào)節(jié)細(xì)胞周期和凋亡相關(guān)蛋白表達(dá)，進而阻滯細(xì)胞周期進程并促進細(xì)胞凋亡有關(guān)。

Objective To investigate the inhibitory effect of allicin combined with cisplatin on the proliferation of human cervical cancer HeLa cells and explore its mechanism. Methods HeLa cells in logarithmic growth phase were set control group (culture solution only), allicin group (50 μg/mL), cisplatin group (5 μg/mL) and combination group (50 μg/mL allicin + 5 μg/mL cisplatin). 48 h after given drugs, the proliferation inhibition rate of HeLa cells was detected by MTT method, the allicin and cisplatin combination index (CI) was calculated by CompuSyn software; the cell cycle distribution and apoptosis level were detected by Flow Cytometry; the expression of Cyclin D1, CDK2, P27, Cleaved Caspase-3, Bcl-2, Bax proteins were detected by Western blotting. Results Compared with allicin group and cisplatin group, the proliferation inhibition rate of combination group was significantly increased (P < 0.05 or 0.01), CI was 0.69 (which suggested that allicin and cisplatin have moderate synergistic effect). Compared with control group, the cell ratio in G₀/G₁ phase and the apoptosis rate in allicin group and cisplatin group were significantly increased (P < 0.05 or 0.01), the expressions of Cyclin D1, CDK2, Bcl-2 were significantly decreased and P27, Cleaved Caspase-3, Bax were significantly increased (P < 0.05 or 0.01), the ratio of Bax/Bcl-2 was significantly increased (P < 0.01). Compared with allicin group and cisplatin group, the cell ratio in G₀/G₁ phase and the apoptosis rate in combination group were significantly increased (P < 0.05 or 0.01), the expressions of Cyclin D1 was significantly decreased and P27, Cleaved Caspase-3, Bax were significantly increased (P < 0.05 or 0.01), the ratio of Bax/Bcl-2 was significantly increased (P < 0.01). Conclusions Allicin combined with cisplatin has the synergistically effects on inhibiting the proliferation of human cervical cancer HeLa cells; which may be related to the regulation of cell cycle and apoptosis-related protein expression, thereby blocking cell cycle progress and promoting cell apoptosis.

R965.1

河北省醫(yī)學(xué)科學(xué)研究重點課題計劃（20181831）