目的 探討香砂平胃顆粒對(duì)濕阻中焦證大鼠胃腸功能的影響及機(jī)制。方法 將大鼠隨機(jī)分成對(duì)照組、模型組、莫沙必利組（1.35 mg/kg）、洛哌丁胺組（0.36 mg/kg）和香砂平胃顆粒低、中、高劑量（0.9、1.8、3.6 g/kg）組，除對(duì)照組外，內(nèi)濕（定期ig豬油、蜂蜜水）、外濕（游泳、放在潮濕墊料中飼養(yǎng)）結(jié)合制備濕阻中焦證動(dòng)物模型，模型成功后，連續(xù)ig給藥7 d。觀察大鼠一般狀態(tài)，采用稱質(zhì)量法計(jì)算大鼠胃殘留率，小腸炭末推進(jìn)法計(jì)算大鼠腸推進(jìn)率，并利用酶聯(lián)免疫吸附法檢測(cè)大鼠血清中胃動(dòng)素、胃泌素、生長(zhǎng)抑素和P-物質(zhì)，以及炎性因子腫瘤壞死因子（TNF）-α和白細(xì)胞介素（IL）-10的水平，同時(shí)利用實(shí)時(shí)熒光定量RCR（qRT-PCR）技術(shù)檢測(cè)大鼠結(jié)腸水通道蛋白3（AQP3）mRNA轉(zhuǎn)錄水平的變化。結(jié)果 與對(duì)照組比較，模型組大鼠體質(zhì)量增長(zhǎng)明顯減緩，攝食量、飲水量均減少，毛色發(fā)黃、乏力、呆滯、嗜睡、大便黏膩；給予香砂平胃顆粒治療7 d后，一般情況有所恢復(fù)，攝食量增加，體質(zhì)量增長(zhǎng)加快，大便性狀有所好轉(zhuǎn)。與模型組比較，香砂平胃顆粒中、高劑量組大鼠胃排空率顯著增加，高劑量組小腸炭墨推進(jìn)率顯著增加（P<0.01、0.001）；中、高劑量組大鼠的胃動(dòng)素、胃泌素和P-物質(zhì)水平顯著增加（P<0.01、0.001），高劑量組生長(zhǎng)抑素水平顯著降低（P<0.01）；中、高劑量組血清TNF-α水平顯著降低（P<0.01、0.001）；高劑量組大鼠的IL-10水平顯著增高（P<0.01）；中、高劑量組大鼠的AQP3mRNA轉(zhuǎn)錄水平顯著上調(diào)（P<0.05、0.001）。結(jié)論 香砂平胃顆?？梢愿纳茲褡柚薪棺C大鼠的胃腸功能，機(jī)制可能與增強(qiáng)胃腸運(yùn)動(dòng)、增加胃腸激素分泌、抑制濕阻所致的消化道炎癥以及促進(jìn)水分從腸腔的轉(zhuǎn)運(yùn)相關(guān)。

Objective To study the effects of Xiangsha Pingwei Keli for the syndrome of damp retention in middle-jiao. Methods The rats were divided into control group, model group, mosapride (1.35 mg/kg) group, loperamide (0.36 mg/kg) group, Xiangsha Pingwei Keli low, middle and high dose (0.9, 1.8, and 3.6 g/kg) group. The rats were treated for 7 days. The gastric residual rate was calculated by weighing method, and the intestinal propulsion rate was calculated by small intestinal charcoal pushing method. The contents of motilin, gastrin, somatostatin, substance P, TNF-α and IL-10 in serum were detected by ELISA. At the same time, the expression of AQP3 mRNA was detected by qRT-PCR. Results Compared with control group, the growth of body weight of rats in model group was significantly slowed down, the amount of food intake and water intake were reduced, the hair color was yellow, fatigue, lethargy, drowsiness, and feces were sticky. After 7 days of treatment with Xiangsha Pingwei Keli, the general condition was somewhat recovered, the food intake increased, the body weight increased faster, and the fecal characteristics were improved. Compare with model group. The gastric residual rate of rats in Xiangsha Pingwei Keli medium and high-dose groups was significantly increased, and the intestinal carbon ink propelling rate in the high-dose group was significantly increased (P<0.01 and 0.001). The levels of motilin, gastrin and P-substance in middle and high dose groups were significantly increased (P<0.01 and 0.001), while the levels of somatostatin in high dose group were significantly decreased (P<0.01). Serum TNF-α level in medium and high dose groups was significantly decreased (P<0.01 and 0.001); The level of IL-10 in high-dose group was significantly increased (P<0.01); The transcription level of AQP3 mRNA in medium and high dose groups was significantly up-regulated (P<0.05 and 0.001). Conclusion The mechanism of Xiangsha Pingwei Keli in the treatment of rats with damp retention in middle-jiao syndrome may be to enhance gastrointestinal motility, increase the secretion of gastrointestinal hormones, inhibit gastrointestinal inflammation and promote water transport from intestinal cavity.

“十三五”國(guó)家重點(diǎn)研發(fā)計(jì)劃項(xiàng)目（2018YFC1602104）