目的 探討羅布麻葉水提取物（WEAVL）對鏈脲佐菌素（STZ）致糖尿病模型小鼠的治療作用。方法 隨機將ICR小鼠分為對照組和WEAVL（2.34 g/kg）組，每天ig給藥1次，連續(xù)30 d，檢測空腹血糖和體質量。ICR小鼠按200 mg/kg ip 2% STZ溶液制備糖尿病模型，造模成功的小鼠按血糖值隨機分為模型組、鹽酸二甲雙胍（陽性藥，130 mg/kg）組和WEAVL低、中、高劑量（0.58、1.17、2.34 g/kg）組，另設對照組，連續(xù)給藥30 d，測定體質量、空腹血糖、糖耐量及血糖曲線下面積（AUC）；稱取肝、腎質量，計算臟器系數(shù)；取胰腺組織進行HE染色、組織病理學檢查；Western blotting檢測腸道組織胰高血糖素樣肽-1（GLP-1）蛋白的表達水平。結果 與對照組比較，單純給予WEAVL對正常動物空腹血糖無明顯影響，可顯著降低小鼠體質量（P<0.01）。與對照組比較，模型組小鼠血糖顯著升高（P<0.01），各組小鼠體質量均顯著降低（P<0.01）；與模型組比較，WEAVL高劑量組給藥第2~4周體質量顯著降低（P<0.05、0.01），3個劑量組2 h血糖、血糖AUC均顯著降低（P<0.01），高劑量組肝臟系數(shù)顯著降低（P<0.05）、腎臟系數(shù)顯著升高（P<0.05），中、高劑量組動物胰島細胞空泡變性例數(shù)減少，中、高劑量組腸道組織GLP-1蛋白的表達顯著上調(diào)（P<0.05）。結論 WEAVL對STZ致糖尿病小鼠有一定治療作用，其作用機制可能與上調(diào)腸道組織中GLP-1的表達有關。

Objective To explore the therapeutic effects of water extract of Apocynum venetum leaves (WEAVL) on STZ-induced diabetic mice model. Methods ICR mice were randomly divided into control group and WEAVL (2.34 g/kg) group. The WEAVL group was given intragastric administration once a day for 30 days, and body mass and FPG was detected. ICR mice were modeled by intraperitoneal injection of 2% STZ solution at 200 mg/kg and 10 mL/kg. The successful models were randomly divided into model group, positive drug group (metformin 130 mg/kg), WEAVL low, medium and high dose (0.58, 1.17 and 2.34 g/kg) group according to blood glucose value. A control group was set up with normal group. After continuous administration for 30 days, the body weight, FPG, OGTT and the area under the curve (AUC) of blood glucose were measured. The liver and kidney was weighed and the organ coefficients were calculated. Pancreatic tissues were HE stained for histopathological examination, Western blotting was used to evaluate the GLP-1 expression in ileum tissue of mice. Results Compared with control group, WEAVL only had no significant effect on FPG in normal animals, and the weight of mice decreased (P<0.01). Compared with the control group, the blood glucose of the model group was increased significantly (P<0.01), and the body weight of the mice in each group was significantly reduced (P<0.01); compared with model group, the body weight of the WEAVL high-dose group was significantly reduced (P<0.05 and 0.01), the two-hour blood glucose and the AUC of blood glucose of the positive drug group and the three dose groups of WEAVL were significantly reduced (P<0.01), the liver body coefficient of the WEAVL high dose group were significantly reduced (P<0.05), and the kidney body coefficient was obvious elevated (P<0.05), the number of cases of vacuolar degeneration in islet cell in the WEAVL middle and high dose groups was less than that in the model group, WEAVL significantly up-regulated the protein expression of GLP-1 in ileum tissue (P<0.05). Conclusions WEAVL has a certain therapeutic effect on STZ-induced diabetic mice, which may be related to the regulation of GLP-1 activation.

R285.5

新疆維吾爾自治區(qū)重大科技專項項目（2016A03006、2016A03006-2、2016A03006-4）