目的 研究五味子乙素聯(lián)合潑尼松對(duì)膜性腎病大鼠的作用及對(duì)其對(duì)PI3K/Akt信號(hào)通路的影響。方法 取15只SD大鼠作為對(duì)照組，60只大鼠采用陽離子化牛血清白蛋白（C-BSA）建立膜性腎病模型，造模完成后的大鼠隨機(jī)分為模型組、潑尼松（2 mg/kg）組、五味子乙素（30 mg/kg）組、五味子乙素（30 mg/kg） ＋潑尼松（2 mg/kg）組。連續(xù)給藥28 d后，分別測定各組大鼠24 h尿蛋白量、血清總膽固醇（TC）、三酰甘油（TG）、丙二醛（MDA）、超氧化物歧化酶（SOD）、尿素氮（BUN）及肌酐（Scr）水平，蘇木精-伊紅染色法進(jìn)行組織病理學(xué)檢查，Western blotting法檢測大鼠腎臟p-Akt、Akt、PI3K-P85、PI3K-P110蛋白表達(dá)水平。結(jié)果 與模型組比較，潑尼松組、五味子乙素組、五味子乙素＋潑尼松組大鼠24 h尿蛋白量和血清TC、TG、MDA、BUN及Scr水平均顯著降低（P<0.05），SOD水平顯著升高（P<0.05），組織病理學(xué)明顯改善，腎臟p-Akt、Akt、PI3K-P85、PI3K-P110蛋白表達(dá)水平均顯著降低（P<0.05），且五味子乙素＋潑尼松組各項(xiàng)指標(biāo)均優(yōu)于單給藥組。結(jié)論 五味子乙素聯(lián)合潑尼松對(duì)大鼠膜性腎病發(fā)揮顯著改善作用，作用優(yōu)于單給藥，其機(jī)制可能與調(diào)節(jié)PI3K/Akt信號(hào)通路有關(guān)。

Objective To study the effect of schisandrin B combined with glucocorticoid on membranous nephropathy in rats and the effect of PI3K/Akt signal pathway in vivo. Methods Tatolly 15 SD rats were selected as the control group, and 60 rats were used to establish membranous nephropathy model with cationic bovine serum albumin (C-BSA). After modeling, the rats were randomly divided into model group, prednisone (2 mg/kg) group, schisandrin B (30 mg/kg) group, schisandrin B (30 mg/kg) + prednisone (2 mg/kg) group. After 28 days of continuous administration, 24-hour urinary protein, serum total cholesterol (TC), triglyceride (TG), malondialdehyde (MDA), total superoxide dismutase (SOD), urea nitrogen (BUN) and creatinine (Scr) were measured. Hematoxylin-eosin staining was used for histopathological examination, Western blotting was used to detect the protein levels of pAkt, Akt, PI3K-P85 and PI3K-P110 in kidney. Results Compared with model group, the levels of 24-hour urinary protein and serum TC, TG, MDA, BUN and Scr in prednisone group, schisandrin B group and schisandrin B + prednisone group were significantly lower than those in model group (P < 0.05). The levels of p-Akt, Akt, PI3K-P85 and PI3K-P110 in kidney were significantly decreased in prednisone group, schisandrin B group and schisandrin B + prednisone group (P < 0.05), and the effect of schisandrin B combined with prednisone group was better than that in prednisone and schisandrin B group. Conclusion Schisandrin B combined with prednisone can significantly improve membranous nephropathy in rats. The mechanism may be related to the regulation of PI3K/Akt signal pathway.

R285.5

湖北省中醫(yī)藥科研立項(xiàng)項(xiàng)目（ZY2019Z014）