[關(guān)鍵詞]
[摘要]
目的 評(píng)價(jià)二甲基姜黃素、黃芩苷、紫檀茋對(duì)異煙肼導(dǎo)致斑馬魚(yú)神經(jīng)損傷的保護(hù)作用。方法 挑選受精后2 h(2hpf)的斑馬魚(yú)胚胎,設(shè)置對(duì)照組(胚胎培養(yǎng)用水處理)、異煙肼組(4 mmol/L)、二甲基姜黃素(0.5、1.0、2.0、4.0 μmol/L)組、黃芩苷(0.1、0.2、0.5、1.0 μmol/L)組、紫檀茋(0.5、1.0、2.0、4.0 μmol/L)組,異煙肼分別與不同濃度的藥物共處理120 h,用Zebrabox斑馬魚(yú)行為分析儀分析斑馬魚(yú)幼魚(yú)行為學(xué)軌跡變化,熒光顯微鏡下觀察斑馬魚(yú)幼魚(yú)多巴胺神經(jīng)元的發(fā)育情況。結(jié)果 與對(duì)照組比較,異煙肼組運(yùn)動(dòng)軌跡明顯減少,運(yùn)動(dòng)總距離和低速、中速運(yùn)動(dòng)距離均顯著下降(P<0.01),運(yùn)動(dòng)速度明顯降低;神經(jīng)節(jié)長(zhǎng)度顯著降低(P<0.01)。與異煙肼組比較,二甲基姜黃素組運(yùn)動(dòng)軌跡明顯增加,斑馬魚(yú)運(yùn)動(dòng)狀態(tài)主要為低速運(yùn)動(dòng),各濃度的二甲基姜黃素組斑馬魚(yú)總運(yùn)動(dòng)距離和低速運(yùn)動(dòng)距離均顯著增加(P<0.01),運(yùn)動(dòng)速度明顯增加,0.5、2.0、4.0 μmol/L的二甲基姜黃素組中速運(yùn)動(dòng)距離顯著增加(P<0.01);黃芩苷組運(yùn)動(dòng)軌跡明顯增加,0.1、0.2、0.5和1.0 μmol/L的黃芩苷組斑馬魚(yú)總運(yùn)動(dòng)距離和低速運(yùn)動(dòng)距離均顯著增加(P<0.01),運(yùn)動(dòng)速度明顯增加;0.5和1.0 μmol/L紫檀茋組運(yùn)動(dòng)軌跡明顯增加,紫檀茋0.5 μmol/L組的總運(yùn)動(dòng)距離和低速運(yùn)動(dòng)距離均顯著增加(P<0.01),紫檀茋0.5和1.0 μmol/L濃度組運(yùn)動(dòng)速度明顯增加。與異煙肼組比較,二甲基姜黃素和黃芩苷各濃度組多巴胺相對(duì)神經(jīng)節(jié)長(zhǎng)度顯著增加(P<0.01);紫檀茋1 μmol/L組相對(duì)神經(jīng)節(jié)長(zhǎng)度顯著增加(P<0.01)。結(jié)論 二甲基姜黃素、黃芩苷和0.5、1.0 μmol/L紫檀茋對(duì)異煙肼導(dǎo)致的斑馬魚(yú)神經(jīng)損傷有明顯保護(hù)作用。
[Key word]
[Abstract]
Objective To evaluate the protective effects of dimethyl curcumin, baclidin and pterostilbene on isoniazid induced nerve damage in zebrafish. Methods 2 hpf (hours post fertilization) zebrafish embryos were used in the experiments. These fish were respectively treated with control group, isoniazid group, and isoniazid along with different concentrations of dimethyl curcumin (0.5, 1.0, 2.0, 4.0 μmol/L), bacitrin (0.1, 0.2, 0.5, 1.0 μmol/L) and pterostilbene (0.5, 1.0, 2.0, 4.0 μmol/L) co-treatment group. At 120 h, the Zebrabox was used to analyze the behavioral trajectory changes of zebrafish, and the development of dopaminergic neurons in zebrafish larvae was observed under a fluorescence microscope. Results Compared with control group, the movement track of isoniazid group was significantly reduced, the total movement distance and the low and medium speed movement distance were significantly decreased (P < 0.01), and the movement speed was significantly reduced, and the ganglion length was significantly decreased (P < 0.01). Compared with isoniazid group, the movement track of dimethylcurcumin group was significantly increased, and the zebrafish movement state was mainly low speed movement. The total movement distance and low speed movement distance of each concentration of dimethylcurcumin group were significantly increased (P < 0.01), and the movement speed was significantly increased, the distance of middle speed movement was significantly increased in dimethylcurcumin 0.5, 2.0, 4.0 μmol/L group (P < 0.01). In the baicalin group, the trajectories increased significantly, the total movement distance and low speed movement distance of zebrafish were significantly increased (P < 0.01) and the movement speed was significantly increased (P < 0.01). The trajectories increased significantly in pterostilbene 0.5 and 1.0 μmol/L group, the total movement distance and low speed movement distance of pterostilbene 0.5 μmol/L group were significantly increased (P < 0.01) and the movement speed was significantly increased in pterostilbene 0.5 and 1.0 μmol/L group. Compared with isoniazid group, the relative ganglion length of dopamine in dimethylcurcumin, baicalin and pterostilbene 1 μmol/L groups was significantly increased (P < 0.01).Conclusion Dimethylcurcumin, baclidin and lower-concentration pterostilbene have obvious protective effects on isoniazid-caused zebrafish nerve injury.
[中圖分類號(hào)]
[基金項(xiàng)目]
山東省優(yōu)秀青年人才基金項(xiàng)目(ZR2020YQ60);齊魯工業(yè)大學(xué)(山東省科學(xué)院)科教產(chǎn)融合創(chuàng)新試點(diǎn)工程項(xiàng)目(2020KJCZD08);濟(jì)南市“高校20條”資助項(xiàng)目(2020GXRC053)