近年來中藥活性物質(zhì)篩選的新技術(shù)發(fā)展迅速，利用“受體-配體”之間的特異性結(jié)合，快速篩選出中藥材里含有的與特定靶點結(jié)合的活性小分子物質(zhì)，再進一步鑒定出活性成分結(jié)構(gòu)。這些技術(shù)對闡明中藥藥效的物質(zhì)基礎(chǔ)和中藥新藥的開發(fā)具有重要意義，是對傳統(tǒng)藥物發(fā)現(xiàn)方法的有利補充。綜述了常用的分子對接技術(shù)、高通量篩選技術(shù)、細胞膜色譜技術(shù)的原理、特點及應(yīng)用進展，以期為中藥活性成分的篩選以及新藥研發(fā)提供參考。

In recent years, new screening techniques of active substances in traditional Chinese medicine have developed rapidly. These techniques use the specific binding between ‘receptor-ligand’ to quickly screen active small molecular substances binding to specific targets in traditional Chinese medicine. Further identification of the structure of active ingredients. It is of great significance to clarify the material basis of the efficacy of traditional Chinese medicine and the development of new drugs of traditional Chinese medicine. It is a beneficial supplement to traditional drug discovery methods. the principle, characteristics, application progress and prospect of molecular docking technology, high-throughput screening technology and cell membrane chromatography technology are reviewed, in order to provide reference for the screening method of active components of traditional Chinese medicine.

天津市科技重大專項與工程（18ZXXYSY00120，18ZXXYSY00020）