目的 考察新綠原酸對宮頸癌HeLa細(xì)胞體外遷移模型和斑馬魚移植瘤模型的影響。方法 MTT法檢測新綠原酸（20、40、80、160、320 μmol/L）對HeLa細(xì)胞增殖的作用；應(yīng)用宮頸癌細(xì)胞HeLa劃痕愈合模型和Transwell實驗考察新綠原酸（28、56、113 μmol/L）對體外遷移作用的影響；同時利用構(gòu)建的綠色熒光蛋白（GFP）的HeLa細(xì)胞系建立斑馬魚移植瘤模型，考察新綠原酸（56、113、226 μmol/L）對體內(nèi)移植瘤模型細(xì)胞遷移的作用；Western blotting法檢測新綠原酸對內(nèi)皮生長因子（EGF）誘導(dǎo)的HeLa細(xì)胞中表皮生長因子受體（EGFR）、p-EGFR蛋白表達的影響。結(jié)果 MTT結(jié)果顯示，新綠原酸在160 μmol/L及以下濃度對HeLa細(xì)胞增殖無明顯影響；與對照組比較，56、113 μmol/L新綠原酸顯著抑制劃痕愈合，抑制率分別為49.3%（P<0.05）和64.1%（P<0.01）；Transwell實驗結(jié)果提示，113 μmol/L新綠原酸具有顯著抑制HeLa細(xì)胞向下層遷移的作用（P<0.05）；與模型組比較，113、226 μmol/L新綠原酸可顯著減少斑馬魚移植瘤模型的HeLa細(xì)胞的遷移面積（P<0.05、0.01）；Western blotting結(jié)果顯示，與EGF組比較，新綠原酸對EGFR表達和磷酸化有明顯抑制作用，56、113 μmol/L濃度組差異顯著（P<0.01）。結(jié)論 在未影響HeLa細(xì)胞增殖的濃度下，新綠原酸具有抑制宮頸癌HeLa細(xì)胞遷移的作用，其作用機制與抑制EGFR的表達有關(guān)。

Objective To investigate the effect of 5-caffeoylquinic acid (5-CQA) on migration of human cervical cancer HeLa cells. Methods MTT assay was used to detect the effect of 5-CQA (20, 40, 80, 160, 320 μmol/L) on the proliferation of HeLa cells. The wound healing model of HeLa cells and Transwell were used to detect the migration of 5-CQA (28, 56, 113 μmol/L) in vitro. At the same time, zebrafish xenograft tumor model was established by using the constructed HeLa cell line of green fluorescent protein (GFP), and the migration of 5-CQA (56, 113, 226 μmol/L) was detected. Western blotting was used to detect the effect of 5-CQA on the expression of p-epidermal growth factor receptor (EGFR) and EGFR protein in HeLa cells induced by EGF. Results MTT results showed that there was no significant effect on the proliferation of HeLa cells of 5-CQA at the concentration of 160 μmol/L or below. Compared with control group, 56, 113 μmol/L 5-CQA significantly inhibited wound healing with inhibition rates of 49.3% (P < 0.05) and 64.1% (P < 0.01), respectively. Transwell results showed that 113 μmol/L 5-CQA significantly inhibited the migration of HeLa cells (P < 0.05). Compared with model group, 113, 226 μmol/L 5-CQA significantly reduced the migration area of HeLa cells (P < 0.05, 0.01). Western blotting results showed that compared with EGF group, 5-CQA (56 and 113 μmol/L) significantly inhibited EGFR expression and phosphorylation (P < 0.01). Conclusions 5-CQA can inhibite cervical cancer HeLa cell migration by blocking EGFR expression.

R285.5;R737.3;R332

國家重點研發(fā)計劃（2018YFC1707300）；山東省重大科技創(chuàng)新工程項目（2019JZZY020905）；齊魯工業(yè)大學(xué)（山東省科學(xué)院）科教產(chǎn)融合創(chuàng)新試點工程項目（2020KJC-ZD08）